A Comprehensive Identification of Chicken Egg White Phosphoproteomics Based on a Novel Digestion Approach
A Comprehensive Identification of Chicken Egg White Phosphoproteomics Based on a Novel Digestion Approach
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基于新型消化方法的鸡蛋清磷酸化蛋白质组学综合鉴定
DOI：
10.1021/acs.jafc.0c03174
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发表时间：
2020-08-26
影响因子：
6.1
通讯作者：
Sheng，Long
中科院分区：
文献类型：
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作者：
Sun，Yi;Jin，Haobo;Sheng，Long
鸡蛋白色（CEW）中含有丰富的磷蛋白，这些磷蛋白对CEW的生物活性和功能具有重要意义。在这项研究中，磷酸化的蛋白质CEW进行了鉴定和分析的基础上两种消化策略（胰蛋白酶和胰蛋白酶/谷氨酰内切蛋白酶）。此外，采用固定化金属亲和层析富集策略，并通过纳米液相色谱/串联质谱鉴定磷酸肽。共鉴定出189个磷酸化位点，对应于96个磷蛋白。基因本体分析表明，这些磷蛋白主要参与“细胞过程”、“生物调节”和“对刺激的反应”等生物学过程。此外，磷蛋白的CEW参与的分子功能，主要包括“结合”和“催化活性”。在现有文献的基础上，本研究首次对鸡蛋白色磷蛋白进行了全面的鉴定。本研究进一步丰富了CEW中磷蛋白的鉴定，为后续磷蛋白的研究奠定了基础。
There are plenty of phosphoproteins in chicken egg white (CEW), which are of great significance for the biological activity and function of CEW. In this study, phosphorylated proteins in CEW were identified and analyzed based on two digestion strategies (trypsin and trypsin/glutamyl endoproteinase). Besides, the enrichment strategy of immobilized metal affinity chromatography was used, and phosphopeptides were identified by nano liquid chromatography/tandem mass spectrometry. A total of 189 phosphosites mapped onto 166 phosphopeptides corresponding to 96 phosphoproteins were identified. Gene ontology analysis suggested that these phosphoproteins of CEW mainly participated in biological processes such as “cell process”, “biological regulation”, and “response to stimulus”. Moreover, the phosphoproteins of CEW were involved in molecular functions, primarily including “binding” and “catalytic activity”. On the basis of the available literature, the research was the first comprehensive identification of chicken egg white phosphoproteins. This study further enriched the identification of phosphoproteins in CEW and laid a foundation for the subsequent study of phosphoproteins.