Functionally distinct genes regulated by hydrogen limitation and growth rate in methanogenic Archaea
Functionally distinct genes regulated by hydrogen limitation and growth rate in methanogenic Archaea
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DOI：
10.1073/pnas.0701157104
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发表时间：
2007-05-22
影响因子：
11.1
通讯作者：
Leigh, John A.
中科院分区：
文献类型：
--
作者：
Hendrickson, Erik L.;Haydock, Andrew K.;Leigh, John A.
利用分子氢作为电子供体来产生能量是氢营养产甲烷菌的一个定义性特征，氢营养产甲烷菌是一个古老的类群，在广古菌门中占主导地位。我们在这里提出了一个全球性的研究mRNA丰度的变化，响应氢营养产甲烷菌的氢可用性。采用连续培养的方法培养海沼甲烷球菌细胞，以消除氢限制和生长速率的影响，并进行微阵列分析。氢限制显着增加基因编码的产甲烷途径，减少或氧化的电子携带脱氮黄素，辅酶F-420的酶的mRNA水平。F-420依赖的氧化还原功能在能量产生代谢的产甲烷菌的特点，结果表明，他们的调节是不同于其他氧化还原过程中的细胞。快速生长增加了一种不寻常的氢化酶基因的mRNA水平，这种氢化酶是氢依赖的亚甲基四氢甲烷蝶呤脱氢酶。
The use of molecular hydrogen as electron donor for energy generation is a defining characteristic of the hydrogenotrophic methanogens, an ancient group that dominates the phylum Euryarchaeota. We present here a global study of changes in mRNA abundance in response to hydrogen availability for a hydrogenotrophic methanogen. Cells of Methanococcus maripaludiswere grown by using continuous culture to deconvolute the effects of hydrogen limitation and growth rate, and microarray analyses were conducted. Hydrogen limitation markedly increased mRNA levels for genes encoding enzymes of the methanogenic pathway that reduce or oxidize the electron-carrying deazaflavin, coenzyme F-420. F-420-dependent redox functions in energy-generating metabolism are characteristic of the methanogenic Archaea, and the results show that their regulation is distinct from other redox processes in the cell. Rapid growth increased mRNA levels of the gene for an unusual hydrogenase, the hydrogen -de pendent methylenetetrahydromethanopterin dehydrogenase.