Inhibition of LDL receptor-related protein 3 suppresses chondrogenesis of stem cells, inhibits proliferation, and promotes apoptosis.
Inhibition of LDL receptor-related protein 3 suppresses chondrogenesis of stem cells, inhibits proliferation, and promotes apoptosis.
复制标题
抑制 LDL 受体相关蛋白 3 可抑制干细胞的软骨形成、抑制增殖并促进细胞凋亡。
DOI：
10.1016/j.bbrc.2022.10.035
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发表时间：
2022
影响因子：
3.1
通讯作者：
Y. Ao
中科院分区：
文献类型：
--
作者：
Yuanyuan Shi;C. Cao;Fan Yang;J. Shao;Xiaoqing Hu;Jin Cheng;Jianquan Wang;Y. Ao
关节软骨缺损仍然是最常见和最具挑战性的关节疾病。软骨由于其无血管性而缺乏损伤后的自愈能力。最近，干细胞疗法被应用于软骨再生。然而，在软骨形成过程中干细胞的关键靶点仍不清楚。我们首先报道了低密度脂蛋白受体相关蛋白3 （LRP3）在干细胞软骨形成过程中的表达显著增加。此外，通过敲低和过表达实验以及RNA测序证实，LRP3是一种有效的软骨生成刺激物。此外，抑制LRP3可抑制细胞增殖，诱导细胞凋亡。因此，我们的研究首先定义了一种新的软骨生成刺激剂LRP3，并进行了详细的澄清，为干细胞软骨再生提供了新的靶点。
Articular cartilage defects remain the most common and challenging joint disease. Cartilage lacks the self-healing capacity after injury due to its avascularity. Recently, stem cell-based therapy has been applied for cartilage regeneration. However, the critical target for stem cells during chondrogenesis remains unclear. We first reported that LDL receptor-related protein 3 (LRP3) expression was markedly increased during chondrogenesis in stem cells. Furthermore, LRP3 was an effective chondrogenic stimulator, as confirmed by knockdown and overexpression experiments and RNA sequencing. In addition, inhibition of LRP3 suppressed proliferation and induced apoptosis. Therefore, our study first defined a new chondrogenic stimulator, LRP3, with detailed clarification, which provided a novel target for stem cell-based cartilage regeneration.