Identification of small auxin-up RNA (SAUR) genes in Urticales plants: mulberry (Morus notabilis), hemp (Cannabis sativa) and ramie (Boehmeria nivea)
Identification of small auxin-up RNA (SAUR) genes in Urticales plants: mulberry (Morus notabilis), hemp (Cannabis sativa) and ramie (Boehmeria nivea)
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DOI：
10.1007/s12041-016-0622-5
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发表时间：
2016-03-01
影响因子：
1.5
通讯作者：
Peng, Dingxiang
中科院分区：
文献类型：
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作者：
Huang, Xing;Bao, Yaning;Peng, Dingxiang
小生长素上调RNA（SAUR）基因是生长素信号转导中的重要基因家族，通常被用作生长素早期应答标记。迄今为止，尽管已发表了桑葚、大麻和苎麻的生物信息，但在荨麻目植物中还没有发现SAUR基因。在这项研究中，我们使用拟南芥的序列作为查询到桑葚，大麻基因组和苎麻转录组数据库。在桑葚、大麻和苎麻中分别获得62、56和71个SAUR基因，系统发育分析表明SAUR基因在荨麻目中特异性扩增，表达分析表明随机选取的15个苎麻SAUR基因在苎麻组织中功能不全，并揭示了一系列IAA响应、干旱响应和高温响应基因。此外，qRT-PCR数据和以前的RNA-Seq数据的比较表明了我们工作的可靠性。在这项研究中，我们首次报告了鉴定的SAUR基因在荨麻目植物。这些结果将为进一步验证其在荨麻目植物生长发育中的功能奠定基础，
Small auxin-up RNA (SAUR) genes are important gene families in auxin signalling transduction and are commonly used as early auxin responsive markers. Till date, no SAUR gene is identified in Urticales plants despite of the published bioinformation of mulberry, hemp and ramie. In this study, we used Arabidopsis sequences as query to search against mulberry, hemp genomes and ramie transcriptome database. In total, we obtained 62, 56 and 71 SAUR genes in mulberry, hemp and ramie, respectively, Phylogenetic analysis revealed the Urticales specific expansion of SAUR genes, Expression analysis showed 15 randomly selected ramie SAUR genes that were diversely functioned in ramie tissues and revealed a series of IAA-responsive, drought-responsive and high temperature-responsive genes. Moreover, comparison of qRT-PCR data and previous RNA-Seq data suggested the reliability of our work. In this study, we first report the identification of SAUR genes in Urticales plants. These results will provide a foundation for their function validation in Urticales plant growth and development,