A Protein Identical to the Yolk Protein Is Stored in the Testis in Male Red Sea Urchin, Pseudocentrotus depressus.
A Protein Identical to the Yolk Protein Is Stored in the Testis in Male Red Sea Urchin, Pseudocentrotus depressus.
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雄性红海胆 Pseudocentrotus depressus 的睾丸中储存着与卵黄蛋白相同的蛋白质。
DOI：
10.2307/1542517
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
T. Akiyama
中科院分区：
文献类型：
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作者：
T. Unuma;T. Suzuki;T. Kurokawa;T. Yamamoto;T. Akiyama
雌性海胆在卵黄形成前将主要卵黄蛋白（MYP）储存在卵巢营养吞噬细胞中。利用免疫学方法，我们检测了红海胆 Pseudocentrotus depressus 睾丸营养吞噬细胞中的 MYP，然后比较了配子发生过程中 MYP 在两性之间的分布。通过离子交换层析（Q Sepharose）和凝胶过滤（Superdex 200）从未受精卵中纯化MYP，并产生针对MYP的抗血清（抗MYP）。免疫印迹分析表明，未成熟的睾丸和卵巢含有大量的 MYP。免疫组织化学显示，无论雄性还是雌性，MYP都分布在占据滤泡腔的营养性吞噬细胞中。在两性中，随着配子发生的进行，营养性吞噬细胞退化，性腺腔充满配子。 MYP 在成熟卵子中作为成熟卵巢中的卵黄蛋白积累。相反，在成熟睾丸中未检测到 MYP，因为储存的精子不与抗 MYP 发生反应。我们得出结论，在雄性 P. depressus 中，MYP 储存在睾丸营养吞噬细胞中，并用作精子发生的营养源。
Female sea urchins store the major yolk protein (MYP) in ovarian nutritive phagocytes before vitellogenesis. Using immunological procedures, we detected MYP in the testicular nutritive phagocytes of Pseudocentrotus depressus, the red sea urchin, and then compared the distribution of MYP between sexes during gametogenesis. MYP was purified from unfertilized eggs by ion exchange chromatography (Q Sepharose) and gel filtration (Superdex 200), and an antiserum (anti-MYP) was raised against MYP. Immunoblot analysis demonstrated that immature testes, as well as ovaries, contained a large quantity of MYP. Immunohistochemistry showed that MYP was distributed in the nutritive phagocytes occupying the follicular lumen in both males and females. In both sexes, as gametogenesis proceeded, the nutritive phagocytes degenerated and the gonadal lumen filled with gametes. MYP accumulated in ripe ova as a yolk protein in the mature ovary. In contrast, MYP was not detected in mature testes, because stored spermatozoa did not react with anti-MYP. We conclude that in male P. depressus, MYP is stored in the testicular nutritive phagocytes and utilized as the nutrient source for spermatogenesis.