Non-invasive, dynamic imaging of murine intestinal motility
Non-invasive, dynamic imaging of murine intestinal motility
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DOI：
10.1111/j.1365-2982.2011.01736.x
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发表时间：
2011-09-01
影响因子：
3.5
通讯作者：
Sevick-Muraca, E. M.
中科院分区：
文献类型：
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作者：
Kwon, S.;Sevick-Muraca, E. M.
背景静脉（i. v.）在给药后，已知吲哚菁绿色（ICG）经由胆道从肝脏分泌到胆汁中，使得能够对肠进行荧光描绘。此外，ICG是近红外（NIR）可激发荧光团，能够为快速NIR荧光成像提供外源对比度。我们试图在注射ICG后使用动态NIR荧光成像来量化肠运动。方法C57 BL 6小鼠在i. v.和皮内（i.d.）分别注射50和10 μ L ICG。还在注射后1小时进行尸检，分离整个胃肠道并暴露于体外荧光成像。关键结果在体内观察并原位证实了载ICG的荧光胆汁分泌到十二指肠。不同模式的肠运动，如蠕动和节段性运动，在体内动态成像。我们的成像数据显示，收缩频率范围为27至35个周期min（-1），蠕动波的传播速度范围为0.82 +/- 0.5至2.04 +/- 1.12 cm s（-1）。结论与推论注射ICG的动态NIR荧光成像可以提供一种用于肠动力障碍或功能障碍的诊断动力测试和用于治疗剂的潜在评估的方法。
Background After intravenous (i.v.) administration, indocyanine green (ICG) is known to be secreted into bile from the liver via the biliary tracts, enabling fluorescent delineation of the intestine. In addition, ICG is a near-infrared (NIR) excitable fluorophore, capable of providing exogenous contrast for rapid NIR fluorescence imaging. We sought to quantify the intestinal motility using dynamic NIR fluorescence imaging after injection of ICG. Methods C57BL6 mice were dynamically imaged immediately before and up to 24 h after i.v. and intradermal (i.d.) injection of 50 and 10 lL of ICG, respectively. Necropsy was also performed 1 h postinjection and the entire gastrointestinal tract was isolated and exposed for ex vivo fluorescence imaging. Key Results The secretion of ICG-laden fluorescent bile into the duodenum was observed in vivo and confirmed in situ. Different patterns of the intestinal motility, such as peristaltic and segmental motions, were dynamically imaged in vivo. Our imaging data showed that the frequency of contractions ranged from 27 to 35 cycles min(-1) and the propagation velocity of peristaltic waves ranged from 0.82 +/- 0.5 to 2.04 +/- 1.12 cm s(-1). Conclusions & Inferences Dynamic NIR fluorescence imaging with injection of ICG can provide a method for diagnostic motility testing for intestinal motility disorders or dysfunction and for potential evaluation of therapeutic agents.