Astaxanthin production by Phaffia rhodozyma and Haematococcus pluvialis:: a comparative study

Astaxanthin production by Phaffia rhodozyma and Haematococcus pluvialis:: a comparative study
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DOI:
10.1007/s00253-007-0889-9
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发表时间:
2007-06-01
影响因子:
5
通讯作者:
Torres-Munoz, J. A.
Torres-Munoz, J. A.
中科院分区:
工程技术2区
文献类型:
--
作者:
Dominguez-Bocanegra, A. R.;Ponce-Noyola, T.;Torres-Munoz, J. A.

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红酵母(Pastifia rhodozyma)和雨生红球藻(Haematococcus pluvialis)被认为是合成虾青素的主要微生物。已经进行了重要的研究努力来确定虾青素合成的最佳条件。当关注于虾青素的生产时,在H.在BAR培养基上,在连续光照(345 μ mol光子m(-2)s(-1))和无通气条件下生长的雨生花。而在椰奶上生长的突变的R1酵母的发酵产生1,850 μ g/g酵母。然而,当观察虾青素生产率时,情况略有不同。用P. rhodozyma得到的图与H.雨生植物最大报告值为170 μ g/g酵母每天与野生酵母菌株和370 μ g/g酵母每天与突变的R1酵母。在H.雨生红酵母(P. rhodozyma)和雨生红酵母(H. pluvialis)在不同的培养基(BG-11或BAR)、光照强度(177 μ mol photon m(-2)s(-1))、通气量等条件下,最大值为290 ~ 428 μ g/g cell/d。雨生,可以比较。这项研究是基于作者以前的工作,其中色素生产已被报道。
Phaffia rhodozyma (now Xanthophyllomyces dendrorhous) and Haematococcus pluvialis are known as the major prominent microorganisms able to synthesize astaxanthin natural pigment. Important research efforts have been made to determine optimal conditions for astaxanthin synthesis. When the focus is on astaxanthin production, the maximal reported value of 9.2 mg/g cell is obtained within H. pluvialis grown on BAR medium, under continuous illumination (345 mu mol photon m(-2) s(-1)) and without aeration. Whereas fermentation by mutated R1 yeast grown on coconut milk produced 1,850 mu g/g yeast. However, when looking at astaxanthin productivity, the picture is slightly different. The figures obtained with P. rhodozyma are rather similar to those of H. pluvialis. Maximal reported values are 170 mu g/g yeast per day with a wild yeast strain and 370 mu g/g yeast per day with mutated R1 yeast. In the case of H. pluvialis, maximal values ranged from 290 to 428 mu g/g cell per day depending on the media (BG-11 or BAR), light intensity (177 mu mol photon m(-2) s(-1)), aeration, etc. The main aim of this work was to examine how astaxanthin synthesis, by P. rhodozyma and H. pluvialis, could be compared. The study is based on previous works by the authors where pigment productions have been reported.