Identification of miRNAs in a Liver of a Human Fetus by a Modified Method
Identification of miRNAs in a Liver of a Human Fetus by a Modified Method
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DOI：
10.1371/journal.pone.0007594
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发表时间：
2009-10-26
期刊：
影响因子：
3.7
通讯作者：
Chen, Huiping
中科院分区：
文献类型：
--
作者：
Liu, Dong;Fan, Jing;Chen, Huiping
背景：miRNAs是一种长度为17-25个核苷酸的RNA分子，在人类细胞中调节基因表达。有研究表明，不同类型的miRNAs参与不同组织的发育。在肝细胞中，有报道的特定类型的miRNAs，调节基因expression.Methods：我们建立了一个人胎肝cDNA文库的修改克隆协议。然后从菌落中分离质粒。经测序和数据库检索后，对miRNAs进行识别。进行RT-PCR和测序以验证检测到的miRNAs。结果：在胎肝组织中发现了一种新的miRNAs，同时还发现了35种已知的miRNAs。其中一些存在于变体中。通过RT-PCR和测序验证所鉴定的miRNAs。结论：我们的研究结果表明，一组特殊的miRNAs可能以协同的方式在胎肝发育中发挥重要作用。
Background: miRNAs are 17-25 nucleotides long RNA molecules that have been found to regulate gene expression in human cells. There are studies showing that different groups of miRNAs are involved in development of different tissues. In hepatocytes there are reported particular types of miRNAs that regulate gene expression.Methods: We established a human fetal liver cDNA library by a modified cloning protocol. Then plasmid isolation from the colonies was performed. After sequencing and database searching, the miRNAs were recognized. RT-PCR and sequencing were carried out to validate the miRNAs detected. Real-time PCR was used to analyze the expression of each miRNA.Results: One novel miRNA was discovered, together with another 35 previously-known miRNAs in the fetal liver. Some of them existed in variants. The miRNAs identified were validated by RT-PCR and sequencing. Quantitative analysis showed that they have variable expression.Conclusion: Our results indicate that a special group of miRNAs may play an important role in fetal liver development in a synergistic manner.