Normal human lymph node T follicular helper cells and germinal center B cells accessed via fine needle aspirations
Normal human lymph node T follicular helper cells and germinal center B cells accessed via fine needle aspirations
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DOI：
10.1016/j.jim.2020.112746
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发表时间：
2020-04-01
影响因子：
2.2
通讯作者：
Crotty, Shane
中科院分区：
文献类型：
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作者：
Havenar-Daughton, Colin;Newton, Isabel G.;Crotty, Shane
生发中心（GC）对于抗体应答的成熟和记忆B细胞的产生至关重要，这些过程形成了长期保护免受病原体侵害的基础。GC仅出现在淋巴组织中，例如淋巴结，并且不存在于血液中。因此，由于健康淋巴结样品的有限可用性，在正常健康条件下，GC B细胞和GC T滤泡辅助（T-FH）细胞在人体中没有得到充分研究。我们使用微创的常规临床程序，淋巴结细针抽吸（LN FNAs），从健康人受试者中获得LN细胞。这项对73个LN的研究表明，人LN FNAs是一种安全可行的免疫学研究技术，并为非炎症条件下的人GC生物学提供了基准。研究结果表明，通过LN FNAs评估GC反应将应用于人类疫苗接种，过敏和自身免疫性疾病的研究。
Germinal centers (GC) are critically important for maturation of the antibody response and generation of memory B cells, processes that form the basis for long-term protection from pathogens. GCs only occur in lymphoid tissue, such as lymph nodes, and are not present in blood. Therefore, GC B cells and GC T follicular helper (T-FH) cells are not well-studied in humans under normal healthy conditions, due to the limited availability of healthy lymph node samples. We used a minimally invasive, routine clinical procedure, lymph node fine needle aspirations (LN FNAs), to obtain LN cells from healthy human subjects. This study of 73 LNs demonstrates that human LN FNAs are a safe and feasible technique for immunological research, and suggests benchmarks for human GC biology under noninflammatory conditions. The findings indicate that assessment of the GC response via LN FNAs will have application to the study of human vaccination, allergy, and autoimmune disease.