RESISTANCE TO FUSARIUM OXYSPORUM 1, a dominant Arabidopsis disease-resistance gene, is not race specific
RESISTANCE TO FUSARIUM OXYSPORUM 1, a dominant Arabidopsis disease-resistance gene, is not race specific
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DOI：
10.1534/genetics.105.042218
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发表时间：
2005-09-01
期刊：
影响因子：
3.3
通讯作者：
Ausubel, FM
中科院分区：
文献类型：
--
作者：
Diener, AC;Ausubel, FM
拟南芥生态型对镰刀菌枯萎病的易感性不同。生态型 Taynuilt-0 (Ty-0) 对尖孢镰刀菌 (f.) matthioli 敏感，而 Columbia-0 (Cot-0) 具有抗性。 Ty-0 和 Col-0 之间杂交的分离分析揭示了 6 个对尖孢镰刀菌 (RFO) 具有显着抗性的位点，这些位点对 f 具有显着贡献。 Col-0 相对于 Ty-0 的 matthioli 抗性。我们将效应最强的基因座称为 RFO1。仅引入 RFO1 的 Col-0 等位基因 (RFO1(col-0)) 的 Ty-0 植物对 f 具有抗性。马蒂奥利。令人惊讶的是，RFO1(Col-0) 也赋予了对 f 的抗性。 raphani，证明 RFO1 介导的耐药性不是种族特异性的。 RFO2、RFO4 或 RFO6 的抗性表达依赖于 RFO1(Col-0)。基于图谱的 RFO1(Col-0) 克隆表明，RFO1 与先前命名的拟南芥基因 WAKL22（壁相关激酶样激酶 22）相同，后者编码不包含细胞外富含亮氨酸重复结构域的受体样激酶。与这些结果一致，Col-0 rfo1 功能丧失突变体更容易受到 f.马蒂奥利，F.球凝素，和f.拉法尼。因此，RFO1 编码一种新型的显性抗病蛋白，赋予对多种镰刀菌属的广谱抗性。
Arabidopsis thaliana ecotypes differ in their susceptibility to Fusarium wilt diseases. Ecotype Taynuilt-0 (Ty-0) is susceptible to Fusarium oxysporum forma specialis (f.) matthioli whereas Columbia-0 (Cot-0) is resistant. Segregation analysis of a cross between Ty-0 and Col-0 revealed six dominant RESISTANCE TO FUSARIUM OXYSPORUM (RFO) loci that significantly contribute to f. matthioli resistance in Col-0 relative to Ty-0. We refer to the locus with the strongest effect as RFO1. Ty-0 plants in which only the Col-0 allele of RFO1 (RFO1(col-0)) was introduced were resistant to f. matthioli. Surprisingly, RFO1(Col-0) also conferred resistance to f. raphani, demonstrating that RFO1-mediated resistance is not race specific. Expression of resistance by RFO2, RFO4, or RFO6 was dependent on RFO1(Col-0). Map-based cloning of RFO1(Col-0) showed that RFO1 is identical to the previously named Arabidopsis gene WAKL22 (WALL-ASSOCIATED KINASE-LIKE KINASE 22), which encodes a receptor-like kinase that does not contain an extracellular leucine-rich repeat domain. Consistent with these results, a Col-0 rfo1 loss-of-function mutant was more susceptible to f. matthioli, f. conglutinans, and f. raphani. Thus, RFO1 encodes a novel type of dominant disease-resistance protein that confers resistance to a broad spectrum of Fusarium races.