Determination of immuno-cross-reactivity between Cryptosporidium parvum and Eimeria spp.
Determination of immuno-cross-reactivity between Cryptosporidium parvum and Eimeria spp.
复制标题
小隐孢子虫和艾美耳球虫之间免疫交叉反应性的测定。
DOI：
10.1016/s0304-4017(97)00225-2
复制
发表时间：
1998
影响因子：
2.6
通讯作者：
M. Ares
中科院分区：
文献类型：
--
作者：
M. Lorenzo;J.A Casal;F. Freire;J.A Castro;C.A Vergara;M. Ares
微小隐孢子虫与艾美耳球虫的免疫交叉反应。用间接荧光抗体试验（IFAT）和Western blot方法对该菌进行了研究。37份来自无症状（非腹泻）牛的血清，已知有关于C.用艾美耳球虫卵囊作抗原，用IFAT法检测了10株Eimeria parvum。大多数血清（54%）显示艾美耳球虫卵囊表面周围的免疫荧光。同时，自然感染艾美耳球虫的家兔血清样品。（E. magna，E.和E. residua），但不含C. parvum感染，用于研究C.通过Western blot程序检测细小卵囊抗原。在11.5-94 kDa范围内的组分以及分子量超过94 kDa的组分可被兔血清识别。在通畅期收集的血清显示与11.5-25 kDa范围内的抗原级分的低或中度反应。然而，29，58和71至75 kDa的蛋白质组分是中度或强烈承认，即使在兔完成卵囊排泄。
Immuno-cross-reactivity between Cryptosporidium parvum and Eimeria spp. was studied by the indirect fluorescent antibody test (IFAT) and Western blot procedure. Thirty-seven sera from asymptomatic (non-diarrheic) cattle, with known coprological (presence–absence of coccidia) and serological data respecting C. parvum, were tested by IFAT using Eimeria oocysts as antigen. Most sera (54%) displayed immunofluorescence around the surface of the Eimeria oocysts. Simultaneously, serum samples from rabbits naturally infected with Eimeria spp. (E. magna, E. intestinalis and E. residua), but free of C. parvum infection, were used to investigate the recognition of C. parvum oocyst antigens by the Western blot procedure. Fractions in the 11.5–94 kDa range, as well as others with molecular masses over 94 kDa, were recognized by sera from rabbits. Sera collected during patency period showed low or moderate reaction with antigenic fractions in the 11.5–25 kDa range. However, 29, 58 and 71 to 75 kDa proteic fractions were moderately or strongly recognized even after rabbits finished oocyst excretion.