A serum shock induces circadian gene expression in mammalian tissue culture cells
A serum shock induces circadian gene expression in mammalian tissue culture cells
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DOI：
10.1016/s0092-8674(00)81199-x
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发表时间：
1998-06-12
期刊：
影响因子：
64.5
通讯作者：
Schibler, U
中科院分区：
文献类型：
--
作者：
Balsalobre, A;Damiola, F;Schibler, U
用高浓度血清处理培养的大鼠成纤维细胞或H35肝癌细胞可诱导多种基因的昼夜节律表达，这些基因的转录在活体动物中也会振荡。振荡基因包括rper1和rper2(果蝇时钟基因周期的大鼠同源基因)，以及编码转录因子Rev-Erbα、DBP和TEF的基因。在RAT-1成纤维细胞中，可以记录到多达三个连续的每日振荡，平均周期长度为22.5小时。在培养细胞和体内，不同的mRNA积累周期的时间顺序是相同的。大鼠成纤维细胞的血清休克还导致c-fos和rper表达的短暂刺激，从而模拟了光诱导的视交叉上核即刻早期基因的表达。
The treatment of cultured rat-1 fibroblasts or H35 hepatoma cells with high concentrations of serum induces the circadian expression of various genes whose transcription also oscillates in living animals. Oscillating genes include rper1 and rper2 (rat homologs of the Drosophila clock gene period), and the genes encoding the transcription factors Rev-Erb alpha, DBP, and TEF. In rat-1 fibroblasts, up to three consecutive daily oscillations with an average period length of 22.5 hr could be recorded. The temporal sequence of the various mRNA accumulation cycles is the same in cultured cells and in vivo. The serum shock of rat-1 fibroblasts also results in a transient stimulation of c-fos and rper expression and thus mimics light-induced immediate-early gene expression in the suprachiasmatic nucleus.