Rapid detection of Mycobacterium tuberculosis cultures by direct ambient corona discharge ionization mass spectrometry of volatile metabolites
Rapid detection of Mycobacterium tuberculosis cultures by direct ambient corona discharge ionization mass spectrometry of volatile metabolites
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通过挥发性代谢物的直接环境电晕放电电离质谱法快速检测结核分枝杆菌培养物
DOI：
10.1039/c6ra12107a
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Ouyang, Yongzhong
中科院分区：
文献类型：
--
作者：
Chingin, Konstantin;Liang, Juchao;Ouyang, Yongzhong
结核病的有效控制在很大程度上依赖于全球人群筛查，但目前的诊断常规不能完全满足结核病识别的特异性和速度要求。本文报道了利用环境电晕放电电离质谱法对细菌培养物释放的挥发性有机化合物（VOCs）进行检测结核分枝杆菌（MTB），这是结核感染的主要原因。MTB初始接种量为104菌落形成单位(CFU) / mL，培养15-20天后可检测到MTB高度特异性的差异挥发性代谢物，如烟酸甲酯和苯甲酸，4-甲氧基-甲酯（lowensten - jensen培养基）。分析的吞吐量约为每分钟3个样品，这比传统技术快得多。总的来说，我们的研究结果表明，环境电晕放电电离质谱法在使用常用的质谱仪器进行快速和经济有效的结核病筛查方面具有很高的适用性。
The effective control of tuberculosis (TB) heavily relies on global population screening, but the current diagnostic routines cannot fully meet the demanded specificity and speed of TB recognition. Here we report on the detection Mycobacterium tuberculosis (MTB), which is the primary cause of TB infection, using ambient corona discharge ionization mass spectrometry analysis of volatile organic compounds (VOCs) emitted by bacterial cultures. For the initial MTB inoculum of 104 colony-forming units (CFU) per mL, highly specific differential volatile metabolites of MTB, such as methyl nicotinate and benzoic acid, 4-methoxy-, methyl ester, could be detected after 15–20 days of incubation (Lowenstein–Jensen medium). The throughput of analysis was ca. 3 samples per minute, which is much faster than in traditional techniques. Overall, our results indicate the high suitability of ambient corona discharge ionization mass spectrometry for the rapid and cost-efficient TB screening using commonly available MS instrumentation.