Rapid biomimetic remineralization of the demineralized enamel surface using nano-particles of amorphous calcium phosphate guided by chimaeric peptides
Rapid biomimetic remineralization of the demineralized enamel surface using nano-particles of amorphous calcium phosphate guided by chimaeric peptides
复制标题
使用嵌合肽引导的无定形磷酸钙纳米颗粒对脱矿牙釉质表面进行快速仿生再矿化
DOI：
10.1016/j.dental.2017.07.015
复制
发表时间：
2017-11-01
期刊：
影响因子：
5
通讯作者：
Zhang, Xu
中科院分区：
文献类型：
--
作者：
Xiao, Z. uohui;Que, Kehua;Zhang, Xu
目标.本研究的目的是开发一种快速有效的方法，使用嵌合肽介导的羧甲基壳聚糖/无定形磷酸钙（CMC/ACP）纳米复合物来矿化人类龋样釉质，模拟釉质生物矿化中由釉原蛋白引导的ACP定向组装的矿化模式。首先通过将羧甲基壳聚糖、氯化钙和磷酸氢二钾依次加入到蒸馏水中来合成CMC/ACP纳米复合物溶液。ACP纳米颗粒被CMC/ACP纳米复合物中的1%NaClO降解。用透射电子显微镜（TEM）对不同时期的颗粒形貌进行了测试。加入嵌合肽以引导ACP纳米颗粒的排列并将ACP纳米颗粒特异性地结合到脱矿釉质表面。采用X射线衍射（XRD）、扫描电子显微镜（SEM）、激光共聚焦显微镜（CLSM）和纳米压痕测试等方法对复合材料的矿化效果进行了研究。得到CMC/ACP纳米复合物，并且可以长时间保持而不沉淀。在NaClO降解和嵌合肽的引导下，ACP纳米颗粒在转化为晶体之前排列成定向阵列，并且釉质样晶体紧密结合在脱矿表面上。新形成的搪瓷状晶体几乎组织良好，并具有很强的机械性能。(C)2017年牙科材料学院。由爱思唯尔有限公司出版。保留所有权利。
Objectives. The objective of this study was to develop a rapid and effective method to remineralize human carious-like enamel using chimaeric peptide-mediated nanocomplexes of carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP), mimicking the mineralizing pattern of the oriented assembly of ACP guided by amelogenin in the biomineralization of enamel.Methods. CMC/ACP nanocomplex solution was first synthesized through the successive addition of carboxymethyl chitosan, calcium chloride, and dipotassium phosphate into distilled water. ACP nanoparticles were degraded by 1% NaClO from CMC/ACP nanocomplexes. The morphology of the particles at different periods was tested by transmission electron microscopy (TEM). The chimaeric peptides were added to guide the arrangement of ACP nanoparticles and to bind ACP nanoparticles to the demineralized enamel surface specifically. X-ray diffraction (XRD)/scanning electron microscope (SEM)/confocal laser scanning microscopy (CLSM)/nano-indentation tests were applied to check the remineralization effects.Results. CMC/ACP nanocomplexes were obtained and could be kept without precipitation for a long time. After the degradation of NaClO and guidance of chimaeric peptides, ACP nanoparticles were arranged into oriented arrays before transforming into crystals, and the enamel-like crystals were tightly bound onto the demineralized surface. The newly formed enamel-like crystals were nearly well-organized and equipped with strong mechanical properties. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.