Biomembrane induced in situ self-assembly of peptide with enhanced antimicrobial activity
Biomembrane induced in situ self-assembly of peptide with enhanced antimicrobial activity
复制标题
生物膜诱导肽原位自组装增强抗菌活性
DOI：
10.1039/c9bm01785b
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发表时间：
2020-04-07
影响因子：
6.6
通讯作者：
Zhang, Yi
中科院分区：
文献类型：
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作者：
Shen, Zhiwei;Guo, Zhen;Zhang, Yi
抗菌肽（AMPs）作为杀菌剂备受关注，因为它们具有对抗抗生素耐药性的能力。通常，天然抗菌肽需要进行合理设计或修饰才能作为抗生素实际应用。在此，设计了一种新型抗菌肽FF8，它是一种由精氨酸、赖氨酸和苯丙氨酸组成的阳离子八肽。研究发现，当受到带负电荷的脂质膜诱导或pH值高于9.4时，FF8会自组装成纳米纤维。膜上的纤维会破坏脂质膜，形成孔隙并显著降低其流动性。FF8还通过显著增加大肠杆菌内外膜的通透性并维持细胞内膜的孔隙，从而导致持续的膜渗漏，表现出增强的抗菌活性。由于其高抗菌活性、细胞相容性和成本效益，FF8是一种很有前景的抗菌材料。
Antimicrobial peptides (AMPs) as biocides are of great interest because they have the ability to combat antibiotic resistance. Normally, natural AMPs need to be rationally designed or modified for practical use as an antibiotic. Here, a novel AMP, termed FF8, which is a cationic octapeptide composed of arginine, lysine, and phenylalanine, was designed. The FF8 was found to self-assemble into nanofibers when induced by a negatively charged lipid membrane or pH is above 9.4. The fibers on the membrane broke the lipid membrane, forming pores and significantly reducing its fluidity. FF8 also exhibited enhanced antibacterial activity by significantly increasing the permeability of the inner and outer membranes of Escherichia coli (E. coli) and maintaining the pores of the inner membrane of cells, which caused continuous membrane leakage. Because of its high antibacterial activity, cytocompatibility, and cost-effectiveness, FF8 is a promising antibacterial material.