LncRNA H19 induced by helicobacter pylori infection promotes gastric cancer cell growth via enhancing NF-κB-induced inflammation
LncRNA H19 induced by helicobacter pylori infection promotes gastric cancer cell growth via enhancing NF-κB-induced inflammation
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DOI：
10.1186/s12950-019-0226-y
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发表时间：
2019-11-26
影响因子：
5.1
通讯作者：
Zhang, Ximei
中科院分区：
文献类型：
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作者：
Zhang, Yifeng;Yan, Jin;Zhang, Ximei
本研究旨在探讨长链非编码RNA（lncRNA）H19在幽门螺杆菌（Helicobacter pylori，H. pylori）。方法采用H19基因在H. pylori+/- GC患者和健康供体（对照）以及GC组织和细胞中的pylori表达通过qRT-PCR检测。CCK-8法检测细胞增殖; Transwell法检测细胞迁移和侵袭能力。通过ELISA测定促炎细胞因子的水平。免疫印迹法测定I κ B α、p-I κ B α和p65蛋白水平。结果H19在H.幽门感染的胃癌组织和细胞。此外，H. pylori促进胃癌细胞的存活、迁移、侵袭和炎症反应。H19过表达促进了H.幽门螺杆菌感染的GC细胞通过增强NF-κ B诱导的炎症。结论LncRNA H19促进H.幽门螺杆菌通过增强NF-κ B诱导的炎症诱导GC细胞生长。
Background The aim of this study was to investigate the role of long non-coding RNA (lncRNA) H19 in gastric cancer (GC) with Helicobacter pylori (H. pylori). Methods H19 expression in peripheral blood from H. pylori+/- GC patients and healthy donors (control) as well as in GC tissues and cells were detected by qRT-PCR. Cell proliferation was evaluated by CCK-8 assay. Cell migration and invasion were evaluated by Transwell assay. The levels of pro-inflammatory cytokines were determined by ELISA. The protein levels of I kappa B alpha, p-I kappa B alpha and p65 were determined by western blotting. Results H19 expression was upregulated in H. pylori-infected GC tissues and cells. Furthermore, H. pylori promoted GC cell viability, migration, invasion and inflammatory response. Moreover, H19 overexpression promoted the proliferation, migration and invasion of H. pylori-infected GC cells via enhancing NF-kappa B-induced inflammation. Conclusions LncRNA H19 promotes H. pylori-induced GC cell growth via enhancing NF-kappa B-induced inflammation.