Nanotube molecular transporters: Internalization of carbon nanotube-protein conjugates into mammalian cells
Nanotube molecular transporters: Internalization of carbon nanotube-protein conjugates into mammalian cells
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DOI：
10.1021/ja0486059
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发表时间：
2004-06-09
影响因子：
15
通讯作者：
Dai, HJ
中科院分区：
文献类型：
--
作者：
Kam, NWS;Jessop, TC;Dai, HJ
探讨了各种功能化碳纳米管与几种类型的人类癌细胞之间的相互作用。我们已经制备了改性的纳米管，并已表明，这些可以衍生的方式，使小分子和蛋白质的附着，后者通过一种新的非共价缔合。功能化的碳纳米管进入非粘附的人类癌细胞以及粘附的细胞系（CHO和3 T3），并且本身是无毒的。虽然荧光蛋白链霉亲和素（MW约60 kD）本身不会进入细胞，但当与纳米管-生物素转运蛋白复合时，它很容易进入细胞，并表现出剂量依赖性细胞毒性。摄取途径与吸附介导的内吞作用一致。利用碳纳米管作为分子转运体可以开发各种货物。纳米管的生物相容性和独特的物理、电学、光学和机械性能为药物、蛋白质和基因递送应用的新材料类别提供了基础。
The interactions between various functionalized carbon nanotubes and several types of human cancer cells are explored. We have prepared modified nanotubes and have shown that these can be derivatized in a way that enables attachment of small molecules and of proteins, the latter through a novel noncovalent association. The functionalized carbon nanotubes enter nonadherent human cancer cells as well as adherent cell lines (CHO and 3T3) and by themselves are not toxic. While the fluoresceinated protein streptavidin (MW ≈ 60 kD) by itself does not enter cells, it readily enters cells when complexed to a nanotube−biotin transporter and exhibits dose-dependent cytotoxicity. The uptake pathway is consistent with adsorption-mediated endocytosis. The use of carbon nanotubes as molecular transporters could be exploited for various cargos. The biocompatibility and unique physical, electrical, optical, and mechanical properties of nanotubes provide the basis for new classes of materials for drug, protein, and gene delivery applications.