Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts
Leptospiral lipopolysaccharide stimulates the expression of toll-like receptor 2 and cytokines in pig fibroblasts
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DOI：
10.1111/asj.12254
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发表时间：
2015-02-01
影响因子：
2
通讯作者：
Isogai, Emiko
中科院分区：
文献类型：
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作者：
Guo, Yijie;Fukuda, Tomokazu;Isogai, Emiko
世界各地的猪都患有钩端螺旋体病，造成严重的经济损失和对人类健康的潜在危害。虽然已知钩端螺旋体内毒素(L-内毒素)参与抗原和宿主细胞之间的免疫反应，但对猪的免疫系统如何应答L-内毒素知之甚少。在此，我们用L-脂多糖刺激猪成纤维细胞，然后用实时定量聚合酶链式反应和Western blotting定量检测两种Toll样受体Toll样受体TLR2和TLR4的基因和蛋白表达水平。结果发现，在L-脂多糖刺激后的24小时内，TLR2的表达显著上调，而TLR4的诱导表达相对较弱。我们还发现，L-内毒素刺激后，髓系分化主要反应基因MYD88、IL-6和IL-8基因的表达明显上调。这些结果提示，猪细胞可通过L-脂多糖刺激激活TLR2而不是TLR4，从而诱导细胞因子的表达。
Pigs throughout the world are afflicted with leptospirosis, causing serious economic losses and potential hazards to human health. Although it has been known that leptospiral lipopolysaccharide (L-LPS) is involved in an immunological reaction between an antigen and a host cell, little is known about how the immune system of pigs can respond to L-LPS. Here, we stimulated pig fibroblasts by L-LPS and then quantitatively measured gene and protein expression levels of two toll-like receptors (TLRs), TLR2 and TLR4, by real-time PCR and Western blotting. As a result, expression of TLR2 was found to be significantly up-regulated within 24h after L-LPS stimulation whereas induction of TLR4 expression was relatively weak. We also revealed that of myeloid differentiation primary response gene 88 (MyD88), interleukin 6 (IL-6) and IL-8 gene expressions were markedly up-regulated by L-LPS stimulation. These results may suggest that the pig cell can activate TLR2 rather than TLR4 by L-LPS stimulation, thereby inducing expression of cytokines.