On-bead fluorescence assay for serine/threonine kinases
On-bead fluorescence assay for serine/threonine kinases
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DOI：
10.1021/ol052125k
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发表时间：
2005-12-08
期刊：
影响因子：
5.2
通讯作者：
Higuchi, T
中科院分区：
文献类型：
--
作者：
Akita, S;Umezawa, N;Higuchi, T
[图]描述了一种用于丝氨酸/苏氨酸激酶的新型基于荧光的测定。碱基介导的磷酸基团的β-消除和含巯基的荧光分子的迈克尔加成允许方便和有效地检测酶活性。该方法可广泛适用于各种丝氨酸/苏氨酸激酶。
[GRAPHIC]A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.