Characterization of a 3.5-kbp plasmid from Helicobacter pylori.
Characterization of a 3.5-kbp plasmid from Helicobacter pylori.
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幽门螺杆菌 3.5-kbp 质粒的表征。
DOI：
10.1006/plas.1995.1030
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发表时间：
1995
期刊：
影响因子：
2.6
通讯作者：
S. McIntire
中科院分区：
文献类型：
--
作者：
J. A. Minnis;T. Taylor;J. Knesek;W. Peterson;S. McIntire
从幽门螺杆菌（HPM 180）中分离出一个3.5kbp的质粒（pHPM 180），并测定了DNA序列。ORF1和ORF2是两个开放阅读框，分别编码54，517和27，629 Da的多肽。确定了核糖体结合和启动子共有序列，以及两个232 bp的直接重复序列和四个22 bp的直接重复序列。pHPM 180 ORF1与5.6kbp的H. pylori质粒。ORF1与6个来自细菌质粒的具有θ型复制子的复制蛋白具有氨基酸同源性。pHPM 180非编码DNA与H. pylori pHPK255，一种通过滚环型机制复制的质粒。核糖核酸酶保护试验表明ORF1在H. pylori HPM180。
A 3.5-kbp plasmid (pHPM180) was isolated from Helicobacter pylori (HPM180) and the DNA sequence was determined. Two open reading frames (ORF1 and ORF2) were identified which could encode polypeptides of 54,517 and 27,629 Da, respectively. Ribosome binding and promoter consensus sequences were identified, as well as two 232-bp direct repeats and four 22-bp direct repeats. DNA sequence homology was found between pHPM180 ORF1 and a 684-base pair HindIII fragment from a 5.6-kbp H. pylori plasmid. ORF1 showed amino acid homology with six replication proteins from bacterial plasmids with theta-type replicons. Additional sequence identity was found between pHPM180 noncoding DNA and a segment of H. pylori pHPK255, a plasmid that replicates via a rolling circle type mechanism. A ribonuclease protection assay determined that ORF1 was transcribed in H. pylori HPM180.