Evaluation of cDNA libraries from different developmental stages of Schistosoma mansoni for production of expressed sequence tags (ESTs).
Evaluation of cDNA libraries from different developmental stages of Schistosoma mansoni for production of expressed sequence tags (ESTs).
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DOI：
10.1093/dnares/4.3.231
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发表时间：
1997-06-30
期刊：
影响因子：
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通讯作者：
Pena, S D
中科院分区：
文献类型：
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作者：
Franco, G R;Rabelo, E M;Pena, S D
利用表达序列标签（EST）技术对曼氏血吸虫不同发育阶段基因表达谱进行了比较研究。共产生1401 EST从7个不同的cDNA文库构建从寄生虫生命周期的四个不同阶段。首先评估文库的质量以用于大规模cDNA测序程序。其中大多数被证明有不到20%的无用克隆和超过50%的新基因。对每个文库的冗余度进行了分析，结果表明，一个成虫cDNA文库由少量的高频基因组成。当比较来自不同文库的EST时，我们可以检测到大多数基因仅存在于单个文库中，但其他基因在一个以上的发育阶段表达，可能代表寄生虫中的管家基因。当只考虑一个基因出现在一个以上的文库，共获得466个独特的基因，对应于427个新的S。mansoni基因。在所有的独特基因中，20.2%与其他生物的基因同源，8.3%与S。mansoni特征基因和71.5%的未知基因。
A comparative study of the gene expression profile in different developmental stages of Schistosoma mansoni has been initiated based on the expressed sequence tag (EST) approach. A total of 1401 ESTs were generated from seven different cDNA libraries constructed from four distinct stages of the parasite life cycle. The libraries were first evaluated for their quality for a large-scale cDNA sequencing program. Most of them were shown to have less than 20% useless clones and more than 50% new genes. The redundancy of each library was also analyzed, showing that one adult worm cDNA library was composed of a small number of highly frequent genes. When comparing ESTs from distinct libraries, we could detect that most genes were present only in a single library, but others were expressed in more than one developmental stage and may represent housekeeping genes in the parasite. When considering only once the genes present in more than one library, a total of 466 unique genes were obtained, corresponding to 427 new S. mansoni genes. From the total of unique genes, 20.2% were identified based on homology with genes from other organisms, 8.3% matched S. mansoni characterized genes and 71.5% represent unknown genes.