Gonadotrophin-releasing hormone (GnRH) release in marmosets I: in vivo measurement in ovary-intact and ovariectomised females.
Gonadotrophin-releasing hormone (GnRH) release in marmosets I: in vivo measurement in ovary-intact and ovariectomised females.
复制标题
狨猴 I 中促性腺激素释放激素 (GnRH) 的释放：卵巢完整和卵巢切除雌性的体内测量。
DOI：
10.1111/j.1365-2826.2007.01534.x
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发表时间：
2007
影响因子：
3.2
通讯作者：
Abbott,DH
中科院分区：
文献类型：
--
作者：
Tannenbaum,PL;Schultz-Darken,NJ;Saltzman,W;Terasawa,E;Woller,MJ;Abbott,DH
首次在新世界灵长类动物中表征了体内下丘脑促性腺激素释放激素（GnRH）释放。一种非终末和可重复的推拉灌注（PPP）技术可靠地测量了清醒普通绒猴的GnRH。19名成年女性（n = 8名卵泡中期卵巢完整; n = 11名卵巢切除）安装了长期颅侧固定器，并在每次PPP治疗前2天将推拉套管临时放置在垂体柄正中隆起（S-ME）内的独特位置。 绒猴经历了1-3次PPP（共32次PPP），持续时间长达12 h。 在PPP期间，这些习惯性绒猴的血浆皮质醇水平没有升高，PPP不会破坏卵巢完整雌性的排卵周期或随后的生育能力。GnRH显示出有组织的释放模式，脉冲每50.0 ± 2.6分钟发生一次，持续25.4 ± 1.3分钟。 GnRH脉冲频率在多个PPP中的个体绒猴中是一致的。GnRH平均浓度、基线浓度和脉冲幅度随插管头端在S-ME内的解剖位置而可预测地变化。GnRH释放增加的特征性反应去甲肾上腺素输注和夜间过渡到熄灯期间突然下降。卵巢完整（卵泡中期）和卵巢切除的绒猴在GnRH释放的任何参数上均无显著差异。总体而言，这些结果表明，PPP可以用来可靠地评估体内GnRH释放在绒猴，并将是一个有用的工具，为未来的研究生殖神经内分泌在这个小灵长类动物。
In vivohypothalamic gonadotrophin‐releasing hormone (GnRH) release was characterised for the first time in a New World primate. A nonterminal and repeatable push‐pull perfusion (PPP) technique reliably measured GnRH in conscious common marmoset monkeys. Nineteen adult females (n = 8 ovary‐intact in the mid‐follicular phase; n = 11 ovariectomised) were fitted with long‐term cranial pedestals, and a push‐pull cannula was temporarily placed in unique locations within the pituitary stalk‐median eminence (S‐ME) 2 days prior to each PPP session. Marmosets underwent 1–3 PPPs (32 PPPs in total) lasting up to 12 h. Plasma cortisol levels were not elevated in these habituated marmosets during PPP, and PPP did not disrupt ovulatory cyclicity or subsequent fertility in ovary‐intact females. GnRH displayed an organised pattern of release, with pulses occurring every 50.0 ± 2.6 min and lasting 25.4 ± 1.3 min. GnRH pulse frequency was consistent within individual marmosets across multiple PPPs. GnRH mean concentration, baseline concentration and pulse amplitude varied predictably with anatomical location of the cannula tip within the S‐ME. GnRH release increased characteristically in response to a norepinephrine infusion and decreased abruptly during the evening transition to lights off. Ovary‐intact (mid‐follicular phase) and ovariectomised marmosets did not differ significantly on any parameter of GnRH release. Overall, these results indicate that PPP can be used to reliably assessin vivoGnRH release in marmosets and will be a useful tool for future studies of reproductive neuroendocrinology in this small primate.