Development and clinical application of near-infrared surgical microscope: Preliminary report
Development and clinical application of near-infrared surgical microscope: Preliminary report
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DOI：
10.1055/s-2001-19929
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发表时间：
2001-12-01
影响因子：
--
通讯作者：
Ohta, T
中科院分区：
文献类型：
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作者：
Kuroiwa, T;Kajimoto, Y;Ohta, T
通过手术显微镜观察近红外荧光可以提供有关深层组织的术中信息。我们研究了静脉注射吲哚菁绿色（ICG）后通过手术显微镜拍摄近红外图像的可能性。我们使用的手术显微镜是Zeiss型号OPMI MD和OPMI CS-NC。我们使用卤素灯作为光源，760-810 nm的带通滤波器作为激发滤波器，800 nm的激光作为外部光源，820-920 nm的带通滤波器作为阻挡滤波器。静脉注射25 mg ICG后，使用Hitachi KP-160照相机通过硬脑膜观察脑表面和硬脑膜窦血管的近红外荧光。通过硬脑膜观察脑表面血管的近红外荧光。静脉图像比动脉图像清晰。硬脑膜窦和桥静脉也清晰可见。这些结果表明，近红外荧光显微镜的临床应用是可能的。然而，从ICG发射的更强的荧光对于这样的显微镜具有实际用途是必要的。
Visualization of near-infrared fluorescence through a surgical microscope can provide intraoperative information about deep-seated tissues. We studied the possibility of taking a near-infrared image through a surgical microscope after intravenous injection of indocyanine green (ICG). The surgical microscopes we used were Zeiss models OPMI MD and OPMI CS-NC. We used a halogen lamp as a light source, a band pass filter of 760-810 nm as an excitation filter, a laser light of 800 nm as an external light source, and a band pass filter of 820-920 nm as a barrier filter. Near-infrared fluorescence of vessels on the brain surface and of the dural sinus was visualized through the dura mater after intravenous injection of 25 mg ICG using a Hitachi KP-160 camera. Near-infrared fluorescence of vessels on the brain surface was observed through the dura mater. Venous images were clearer than were arterial ones. The dural sinus and the bridging veins were also clearly visualized. These results suggest that a clinical application of near-infrared fluorescence microscopy is possible. However, a stronger fluorescence emitted from ICG will be necessary for such a microscope to have practical use.