Involvement of hepatocyte growth factor in kidney development.
Involvement of hepatocyte growth factor in kidney development.
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肝细胞生长因子参与肾脏发育。
DOI：
10.1006/dbio.1994.1169
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发表时间：
1994
影响因子：
2.7
通讯作者：
Nigam,SK
中科院分区：
文献类型：
--
作者：
Santos,OF;Barros,EJ;Yang,XM;Matsumoto,K;Nakamura,T;Park,M;Nigam,SK
采用PCR技术分析了小鼠肾脏发育过程中肝细胞生长因子（HGF）和c-met（HGF受体）的表达。HGF和c-met均从孕11.5天开始表达，此时输尿管芽的分支形态发生。将胚胎肾与接种在I型胶原中的MDCK细胞共培养诱导MDCK细胞的分支形态发生。当在培养基中加入抗HGF的单特异性抗血清时，胚胎肾诱导的分支形态发生被显著抑制（73%）。在器官培养试验中，这种抗HGF血清与11.5- 12.5天的胚胎肾脏孵育时也抑制后肾生长。未观察到非免疫血清的抑制作用。在用抗HGF血清孵育的胚胎肾中观察到输尿管发育的扰动以及导管外后肾发育。总之，我们的数据表明HGF在肾脏发育中的重要作用。
Using PCR, hepatocyte growth factor (HGF) and c-met (HGF receptor) expression were analyzed in the developing mouse kidney. Both HGF and c-met were expressed from Gestational Day 11.5 onward, the time at which branching morphogenesis of ureteric bud occurs. Coculturing the embryonic kidney with MDCK cells seeded in Type I collagen induced branching morphogenesis of the MDCK cells. When a monospecific antiserum against HGF was included in the culture medium, the branching morphogenesis induced by the embryonic kidney was markedly inhibited (73%). This anti-HGF serum also inhibited metanephric growth when incubated with 11.5- to 12.5-day embryonic kidneys in an organ culture assay. No inhibition was observed by nonimmune serum. Perturbation of ureteric duct development as well as extraductal metanephric development was observed in embryonic kidneys incubated with the anti-HGF serum. Together, our data indicates an important role for HGF in kidney development.