A potent novel anti-HIV protein from the cultured cyanobacterium Scytonema varium
A potent novel anti-HIV protein from the cultured cyanobacterium Scytonema varium
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DOI：
10.1021/bi0205698
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发表时间：
2003-03-11
期刊：
影响因子：
2.9
通讯作者：
Boyd, MR
中科院分区：
文献类型：
--
作者：
Bokesch, HR;O'Keefe, BR;Boyd, MR
从培养的蓝藻 Scytonema varium 的水提取物中分离出一种新的抗 HIV 蛋白，scytovirin。该蛋白对 HIV-1 实验室菌株和初级分离株表现出有效的抗细胞病变活性，EC50 值范围为 0.3 至 22 nM。 Scytovirin 与病毒外壳蛋白 gp120、gp160 和 gp41 结合，但不与细胞受体 CD4 或其他测试蛋白结合。这种独特的蛋白质由一条 95 个氨基酸的链（具有显着的内部序列重复）和 10 个半胱氨酸组成，形成 5 个链内二硫键。
A new anti-HIV protein, scytovirin, was isolated from aqueous extracts of the cultured cyanobacterium Scytonema varium. The protein displayed potent anticytopathic activity against laboratory strains and primary isolates of HIV-1 with EC50 values ranging from 0.3 to 22 nM. Scytovirin binds to viral coat proteins gp120, gp160, and gp41 but not to cellular receptor CD4 or other tested proteins. This unique protein consists of a single 95-amino acid chain with significant internal sequence duplication and 10 cysteines forming five intrachain disulfide bonds.