Inhibition of influenza virus replication by phosphorothioate and liposomally endocapsulated oligonucleotides.
Inhibition of influenza virus replication by phosphorothioate and liposomally endocapsulated oligonucleotides.
复制标题
硫代磷酸酯和脂质体内包寡核苷酸抑制流感病毒复制。
DOI：
10.1080/07328319808005191
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
H. Takaku
中科院分区：
文献类型：
--
作者：
T. Abe;T. Hatta;K. Takai;H. Nakashima;T. Yokota;H. Takaku
我们已经证明，反义硫代磷酸寡核苷酸（S-ODNs）抑制流感病毒A在MDCK细胞中的复制。合成了具有两个靶位点（PB 1和PB 2）的硫代磷酸酯和脂质体包封的寡核苷酸，并使用MDCK细胞通过MTT测定法测试了病毒诱导的致细胞病变作用。与PB 2-AUG起始密码子互补的脂质体包裹的S-ODNs显示出高度的抑制作用。另一方面，与PB 2靶位点相比，脂质体包封的靶向PB 1的S-ODNs的抑制作用显著降低。脂质体包封的寡核苷酸表现出比游离寡核苷酸更高的抑制活性。脂质体载体的使用有效地提高了修饰的寡核苷酸的活性。
We have demonstrated that antisense phosphorothioate oligonucleotides (S-ODNs) inhibit influenza virus A replication in MDCK cells. Phosphorothioate and liposomally encapsulated oligonucleotides with two target sites (PB1 and PB2) were synthesized and tested for virus-induced cytopathogenicity effects by a MTT assay using MDCK cells. The liposomally encapsulated S-ODNs complementary to the sites of the PB2-AUG initiation codon showed highly inhibitory effects. On the other hand, the inhibitory effect of the liposomally encapsulated S-ODNs targeted to PB1 was considerably decreased in comparison with the PB2 target sites. The liposomally encapsulated oligonucleotides exhibited higher inhibitory activity than the free oligonucleotides. The activities of the modified oligonucleotides are effectively enhanced by using the liposomal carrier.