Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro
Dairy protein and leucine alter GLP-1 release and mRNA of genes involved in intestinal lipid metabolism in vitro
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DOI：
10.1016/j.nut.2008.08.012
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发表时间：
2009-03-01
期刊：
影响因子：
4.4
通讯作者：
Reimer, Raylene A.
中科院分区：
文献类型：
--
作者：
Chen, Qixuan;Reimer, Raylene A.
目的：越来越多的证据支持乳制品的抗肥胖作用；然而，其机制尚不清楚。这项研究的目的是探索乳制品具有抗肥胖作用的可能的肠道机制。以人肠道细胞株NCI-H716为研究对象，验证了支链氨基酸和乳蛋白调节饱腹感激素分泌和调节脂肪酸和胆固醇代谢相关基因的假说。方法：在剂量效应研究中(0.5%、1.0%、2.0%和3.0%)，检测亮氨酸、异亮氨酸、缬氨酸、脱脂牛奶、酪蛋白和乳清对胰高血糖素样肽-1释放的影响以及所选基因的表达。结果：亮氨酸、异亮氨酸、脱脂牛奶和酪蛋白刺激胰高血糖素样肽-1释放(P<；0.05)。异亮氨酸和乳清下调肠型脂肪酸结合蛋白(I-FABP)、脂肪酸转运蛋白4(FATP4)、尼曼-Pick C-1样蛋白(NPC1L1)、乙酰辅酶A羧基酶(ACC)、脂肪酸合成酶(Fas)、固醇调节元件结合蛋白-2(SREBP-2)和3-羟基-3-甲基戊二酰辅酶A还原酶(HMGCR；P<；0.05)的表达。亮氨酸和缬氨酸可下调NPC1L1、A CC、Fas、SREBP-2和HMGCR的表达(P<；0.05)。酪蛋白下调I-FABP、FATP4、ACC、Fas、SREBP-2和HMGCR的表达(P<；0.05)。脱脂牛奶下调ACC、Fas和SREBP-2的表达，但不下调I-FABP、FATP4和NPC1L1的表达。结论：乳制品的减肥作用可能至少部分是通过整合促进高血糖素样多肽-1分泌和抑制肠道脂肪酸和胆固醇吸收合成相关基因的表达而实现的。(C)2009年，爱思唯尔公司出版。
Objective: A growing body of evidence supports an antiobesity effect of dairy products; however, the mechanisms remain unclear. The objective of this study was to explore possible intestinal mechanisms by which dairy delivers an antiobesity effect. The human intestinal cell line, NCI-H716, was used to test the hypothesis that branched-chain amino acids and dairy proteins regulate satiety hormone secretion and modulate genes involved in fatty acid and cholesterol metabolism.Methods: In dose-response (0.5%, 1.0%, 2.0%, and 3.0%) studies, the effect of leucine, isoleucine, valine, skim milk, casein, and whey on glucagon-like peptide-1 release and the expression of selected genes were tested.Results: Leucine, isoleucine, skim milk, and casein stimulated glucagon-like peptide-1 release (P < 0.05). Isoleucine and whey downregulated the expression of intestinal-type fatty acid binding protein (i-FABP), fatty acid transport protein 4 (FATP4), Niemann-Pick C-1-like-1 protein (NPC1L1), acetyl-coenzyme A carboxylase (ACC), fatty acid synthase (FAS), sterol regulatory element-binding protein-2 (SREBP-2), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR; P < 0.05). Leucine and valine downregulated the expression of NPC1L1, A CC, FAS, SREBP-2, and HMGCR (P < 0.05). Casein downregulated the expression of i-FABP, FATP4, ACC, FAS, SREBP-2, and HMGCR (P < 0.05). Skim milk downregulated the expression of ACC, FAS, and SREBP-2, but not i-FABP, FATP4, and NPC1L1.Conclusion: This work suggests that the antiobesity effect of dairy may be mediated, at least in part, by integration of events that promote glucagon-like peptide-1 secretion and inhibit expression of genes involved in intestinal fatty acid and cholesterol absorption and synthesis. (C) 2009 Published by Elsevier Inc.