Studies of glucosidase activities from surface sediments in mangrove swamp
Studies of glucosidase activities from surface sediments in mangrove swamp
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红树林沼泽表层沉积物葡萄糖苷酶活性研究
DOI：
10.1016/j.jembe.2008.09.008
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发表时间：
2008-12-15
影响因子：
2
通讯作者：
Hong, HuaSheng
中科院分区：
文献类型：
--
作者：
Liu, HuiJie;Tian, Yun;Hong, HuaSheng
在九龙江口福贡红树林（117 ° 54 '~ 117 ° 55' E，24 ° 22'24 ° 24' N）建立了4条样带16个站位。除了地球化学特征和细菌丰度的估计，α-和β-葡萄糖苷酶活性的分布进行了研究，以探索碳水化合物的降解，预计会发生在大量的红树林系统。为了更好地了解红树林沉积物中微生物α-葡萄糖苷酶和β-葡萄糖苷酶的活性及其与细菌生物量、代谢活性和环境因子的关系，采用荧光模式底物（FMS）技术研究了红树林沉积物中微生物α-葡萄糖苷酶和β-葡萄糖苷酶活性的分布格局。结果表明，不同站位的α-葡萄糖苷酶（10.839与100.86 μ mol g（-1）h（-1）相似）和β-葡萄糖苷酶（39.60与222.75 μ mol g（-1）h（-1）相似）的酶活性存在差异，各站位β-葡萄糖苷酶的酶活性均高于α-葡萄糖苷酶。胞外酶活性与有机碳、有机质和细菌丰度呈正相关。此外，利用FMS技术测定红树林沉积物的胞外酶活性有助于我们评价其分解代谢行为，从而更好地了解细菌在红树林沼泽生态系统物质循环中的作用。(C)2008 Elsevier B. V.保留所有权利。
Four transects including sixteen stations were established in the Fugong mangrove (117 degrees 54'-117 degrees 55'E, 24 degrees 22'24 degrees 24'N) of the Jiulong River Estuary, Fujian, China. Besides geochemical characterization and estimation of bacterial abundances, the distribution of alpha- and beta-glucosidase activity was studied to explore the degradation of carbohydrates which can be expected to occur in high quantities in mangrove systems. The distribution pattern of microbial alpha-glucosidase and beta-glucosidase activities was investigated using a fluorogenic model substrate (FMS) technique in order to allow better understanding of in situ enzyme activities, as well as their relation to bacterial biomass, metabolic activity and environmental factors in mangrove sediments. The results showed that the enzyme activities of alpha-glucosidase (10.839 similar to 100.86 mu mol g(-1) h(-1)) and beta-glucosidase (39.60 similar to 222.75 mu mol g(-1) h(-1)) varied among the different stations, and the enzyme activities of beta-glucosidase were higher than those of alpha-glucosidase at all stations. The extracellular enzyme activities were positively related to organic C, organic matter and bacterial abundance. In addition, the use of the FMS technique to measure extracellular enzyme activities of mangrove sediments could help us to evaluate their catabolic behavior in situ and so lead to a better understanding of the bacterial role in material cycle of mangrove swamp ecosystems. (C) 2008 Elsevier B.V. All rights reserved.