Derivation of iPSCs in stirred suspension bioreactors
Derivation of iPSCs in stirred suspension bioreactors
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DOI：
10.1038/nmeth.1973
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发表时间：
2012-05-01
期刊：
影响因子：
48
通讯作者：
Rancourt, Derrick E.
中科院分区：
文献类型：
--
作者：
Shafa, Mehdi;Day, Brad;Rancourt, Derrick E.
诱导多能干细胞 (iPSC) 通常来源于贴壁培养。在这里，我们报告了在搅拌悬浮生物反应器中使用或不使用 c-Myc 快速有效地衍生小鼠 iPSC。悬浮重编程细胞表达多能性标记，在体外和体内表现出多谱系分化，并有助于嵌合小鼠的种系。悬浮重编程有可能加速和标准化 iPSC 研究。
Induced pluripotent stem cells (iPSCs) are typically derived in adherent culture. Here we report fast and efficient derivation of mouse iPSCs in stirred suspension bioreactors, with and without the use of c-Myc. Suspension-reprogrammed cells expressed pluripotency markers, showed multilineage differentiation in vitro and in vivo, and contributed to the germline in chimeric mice. Suspension reprogramming has the potential to accelerate and standardize iPSC research.