Increased IL-22 level in allergic rhinitis significantly correlates with clinical severity
Increased IL-22 level in allergic rhinitis significantly correlates with clinical severity
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DOI：
10.2500/ajra.2014.28.4088
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发表时间：
2014-11-01
影响因子：
2.6
通讯作者：
Li, Huabin
中科院分区：
文献类型：
--
作者：
Tang, Jun;Xiao, Ping;Li, Huabin
背景：IL-22调节多种过程，并与多种效应有关。然而，IL-22在变应性鼻炎（AR）发病机制中的重要性仍然知之甚少。本研究旨在评估AR患者IL-22和IL-17A水平及其与AR临床严重程度的关系。方法：选取36例AR患者和22例正常人作为研究对象。用流式细胞术检测AR患者和正常人外周血中IL-22（+）、IL-17A（+）、IL-9(+) T辅助细胞（Th）的频率。采用ELISA法检测AR患者及正常人血清IL-22、IL-17A水平。评估IL-22（+）和IL-17A(+) Th细胞百分比以及血清IL-22和IL-17A水平的临床相关性。结果：与正常对照组相比，IL-22（+）和IL-17A(+) Th细胞频率显著升高，IL-9(+) Th细胞频率无显著升高（p < 0.05）。AR患者外周血IL-22（+）、IL-17A(+) Th细胞频率与鼻症状（鼻塞、鼻漏）视觉模拟评分显著相关，p < 0.05。AR患者血清IL-22、IL-17A水平较正常对照组显著升高（p < 0.05），且与翼状棘球蚴、粉状棘球蚴特异性IgE水平显著相关。结论：IL-22和IL-17A可能在AR患者th2偏斜炎症的调节中发挥重要作用。
Backgroud: IL-22 regulates various processes and has been linked to diverse effects. However, the importance of IL-22 in the pathogenesis of allergic rhinitis (AR) remains poorly understood. This study sought to evaluate the levels of IL-22 and IL-17A in AR patients and their association with clinical severity of AR.Methods: Thirty-six AR patients and 22 normal controls were enrolled in this study. The frequencies of IL-22(+), IL-17A(+), and IL-9(+) T helper (Th) cells in peripheral blood of AR patients and normal controls were examined by flow cytometry. Serum levels of IL-22 and IL-17A in AR patients and normal controls were determined by ELISA. The clinical relevance of the percentages of IL-22(+) and IL-17A(+) Th cells as well as serum IL-22 and IL-17A levels were evaluated.Results: The frequencies of IL-22(+) and IL-17A(+) Th cells, but not IL-9(+) Th cells, were significantly increased compared with those in normal controls (p < 0.05). Frequencies of IL-22(+) and IL-17A(+) Th cells in peripheral blood of AR patients significantly correlated with visual analog scale scores of nasal symptoms (nasal congestion and rhinorrhea; p < 0.05). Moreover, the serum levels of IL-22 and IL-17A were significantly increased compared with those in normal controls (p < 0.05) and significantly correlated with the levels of Dermatophagoides pteronyssinus and Dermatophagoides farinae specific IgE in AR patients.Conclusion: Our findings suggested that IL-22 as well as IL-17A may play an important role in the regulation of Th2-skewed inflammation in AR patients.