Success rates in isolating mesenchymal stem cells from permanent and deciduous teeth
Success rates in isolating mesenchymal stem cells from permanent and deciduous teeth
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DOI：
10.1038/s41598-019-53265-4
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发表时间：
2019-11-14
影响因子：
4.6
通讯作者：
Tanimoto, Kotaro
中科院分区：
文献类型：
--
作者：
Nakajima, Kengo;Kunimatsu, Ryo;Tanimoto, Kotaro
来自人类脱落乳牙（SHED）和人类牙髓干细胞（hDPSC）的干细胞已经成为骨再生的有吸引力的细胞来源。然而，具体的牙齿和最适合干细胞分离的条件仍然不清楚。因此，评估SHED和hDPSCs分离的成功率、患者年龄和乳牙中的剩余牙根长度。成功分离定义为细胞培养物保持至第三次传代，无任何污染或其他问题。剩余牙齿长度的计算使用根冠比从病人的X射线和比较的标准值从文献中。SHED和hDPSC的总体成功分离率分别为82%和70%。乳牙拔除时平均年龄11岁9个月，恒牙拔除时平均年龄22岁10个月。在成功的SHED组中，前乳牙的平均剩余牙根长度为71.4%，乳磨牙的平均剩余牙根长度为61.4%。成功的隔离似乎与患者年龄、剩余牙根的长度以及机械应力和其他因素有关。需要确定牙齿选择标准以提高成功率。
Stem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (hDPSCs) have emerged as attractive cell sources for bone regeneration. However, the specific teeth and the conditions most suitable for stem cell isolation remain unclear. Therefore, the success rate of SHED and hDPSCs isolation, the patient age and remaining root length in deciduous teeth were evaluated. Successful isolation was defined as when the cell culture was maintained up to the third passage without any contamination or other issues. Remaining tooth length was calculated using the root-to-crown ratio from patient X-rays and compared to the norm value from the literature. The overall successful isolation rate of SHED and hDPSCs was 82% and 70%. The average patient ages at extraction of the deciduous teeth and permanent teeth were 11 years and 9 months, and 22 years and 10 months respectively. In the successful SHED group, the average remaining root length of the anterior deciduous teeth was 71.4%, and that of the deciduous molars was 61.4%. Successful isolation appears to be associated with patient age, length of the remaining root, and also mechanical stress and other factors. Tooth selection criteria need to be identified to improve the success rate.