Oral supplementation with melon superoxide dismutase extract promotes antioxidant defences in the brain and prevents stress-induced impairment of spatial memory
Oral supplementation with melon superoxide dismutase extract promotes antioxidant defences in the brain and prevents stress-induced impairment of spatial memory
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DOI：
10.1016/j.bbr.2008.12.038
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发表时间：
2009-06-08
影响因子：
2.7
通讯作者：
Mikami, Toshio
中科院分区：
文献类型：
--
作者：
Nakajima, Sanae;Ohsawa, Ikuroh;Mikami, Toshio
本研究的目的是探讨抗氧化剂摄入对应激引起的认知记忆障碍的影响。雄性C57 BL/6小鼠分为4组：（1）正常饲养组（C组），束缚应激组（RS组），维生素E组（VE组），维生素E组（VE组），对照组（C组），束缚应激组（RS组），维生素E组（VE组），维生素E组（VE组）。（4）GliSOD in小鼠（GS小鼠），在小笼中喂食补充有GliSOD in的饮食。RS，VE和CS小鼠每天暴露于12小时的制动。五周后，使用Morris水迷宫（MWM）测试测量空间学习能力。水迷宫测试后，我们使用4-羟基-2-壬烯醛（4-HNE）和抗Ki 67抗体进行免疫组织化学分析。4-HNE是脂质过氧化的标志物。RS小鼠表现出受损的空间学习能力和海马齿状回的颗粒细胞层（GCL）中的4-HNE阳性细胞的数量增加时，与C小鼠相比。此外，RS小鼠在颗粒下区（SGZ）中的Ki 67阳性细胞数量减少。GS小鼠表现出更好的空间学习记忆比RS小鼠。GS小鼠GCL中4-HNE阳性细胞数明显少于RS小鼠。GS小鼠SGZ中Ki 67阳性细胞的数量显著高于RS小鼠。这些发现表明，GliSODin通过抗氧化活性防止应激诱导的认知功能损伤并维持海马中的神经发生。(C)2008 Elsevier B. V.保留所有权利。
The purpose of this study was to investigate the effect of antioxidant ingestion on stress-induced impairment of cognitive memory. Male C57BL/6 mice were divided into four groups as follows: (1) control mice (C mice) fed in a normal cage without immobilization; (2) restraint-stressed (RS mice) fed in a small cage: (3) vitamin E mice (VE mice), mice were fed in a small cage with a diet supplemented with vitamin E; (4) GliSODin mice (GS mice) fed in a small cage with a diet supplemented with GliSODin. RS, VE and CS mice were exposed to 12 h of immobilization daily. Five weeks later, spatial learning was measured using the Morris Water Maze (MWM) test. After water maze testing, we performed immunohistochemical analysis using 4-hydroxy-2-noneral (4-HNE) and an anti-Ki67 antibody. 4-HNE is a marker of lipid peroxidation. RS mice showed impaired spatial learning performance and an increased number of 4-HNE-positive cells in the granule cell layer (GCL) of the hippocampal dentate gyrus when compared to C mice. Moreover, RS mice showed a decreased number of Ki67-positive cells in the subgranular zone (SGZ). GS mice showed better spatial learning memory than RS mice. The number of 4-HNE-positive cells in the GCL of GS mice was significantly less than that of RS mice. The number of Ki67-positive cells in the SGZ of GS mice was significantly greater than that of RS mice. These finding suggests that GliSODin prevents stress-induced impairment of cognitive function and maintains neurogenesis in the hippocampus through antioxidant activity. (C) 2008 Elsevier B.V. All rights reserved.