Expression of the zic1, zic2, zic3, and zic4 genes in early chick embryos.
Expression of the zic1, zic2, zic3, and zic4 genes in early chick embryos.
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DOI：
10.1186/1756-0500-3-167
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发表时间：
2010-06-16
影响因子：
1.8
通讯作者：
Merzdorf CS
中科院分区：
文献类型：
--
作者：
McMahon AR;Merzdorf CS
zic基因编码的转录因子家族在早期发育过程中发挥重要作用。由于对鸡胚中zic基因的表达知之甚少，我们对神经胚形成和体节发生过程中zic 1、zic 2、zic 3和zic 4（zic 1 -4）基因的表达模式进行了研究。我们使用原位杂交技术分析了zic 1 -4基因在鸡早期发育（HH阶段7-19）过程中的表达模式。ZIC 1 -3基因在背神经管和体节的背侧部分表现出重叠和沿着的基因特异性表达模式。此外，zic基因的独特表达结构域包括：zic 2在神经板，耳周中胚层和肢芽; zic 3在神经板周围的近轴中胚层，在前体中胚层和最近形成的上皮体节; zic 2和zic 3在发育中的眼睛。ZIC 4表达局限于背侧前脑和中脑区域，并且与ZIC 1 -3基因的表达不同，在后脑和躯干中未检测到ZIC 4表达。这与其他脊椎动物中更广泛的zic 4表达形成对比。zic 1 -3基因在神经管、体节和其他外胚层和中胚层衍生的结构中的重叠和独特结构域中表达。然而，ZIC 4表达仅限于未来脑的背前部区域。这是首次对鸡胚神经胚形成和体节发生过程中zic 1 -4基因表达的全面研究。
The zic genes encode a family of transcription factors with important roles during early development. Since little is known about zic gene expression in chick embryos, we have characterized the expression patterns of the zic1, zic2, zic3, and zic4 (zic1-4) genes during neurulation and somitogenesis. We used in situ hybridization to analyze the expression patterns of the zic1-4 genes during early chick development (HH stages 7-19). The zic1-3 genes showed both overlapping and gene-specific expression patterns along the length of the dorsal neural tube and in the dorsal parts of the somites. In addition, unique expression domains of zic genes included: zic2 in the neural plate, periotic mesoderm and limb buds; zic3 in the paraxial mesoderm surrounding the neural plate, in presomitic mesoderm and in the most recently formed epithelial somites; zic2 and zic3 in developing eyes. zic4 expression was limited to dorsal fore- and midbrain regions and, unlike the expression of the zic1-3 genes, zic4 expression was not detected in the hindbrain and trunk. This was in contrast to more extensive zic4 expression in other vertebrates. The zic1-3 genes were expressed in both overlapping and unique domains within the neural tube, somites and other ectoderm and mesoderm-derived structures in the future head and trunk. zic4 expression, however, was limited to dorso-anterior regions of the future brain. This is the first comprehensive study of zic1-4 gene expression in chick embryos during neurulation and somitogenesis.