Ti4+-immobilized hierarchically porous zirconium-organic frameworks for highly efficient enrichment of phosphopeptides
Ti4+-immobilized hierarchically porous zirconium-organic frameworks for highly efficient enrichment of phosphopeptides
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DOI：
10.1007/s00604-021-04760-x
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发表时间：
2021-04
影响因子：
5.7
通讯作者：
Yanting He;Qiong Zheng;Zian Lin
中科院分区：
文献类型：
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作者：
Yanting He;Qiong Zheng;Zian Lin
制备了Ti4+固定的多级多孔锆-有机骨架（THZr-MOFs），用于磷酸肽的富集。THZr-MOFs具有185.28 m2 g-1的高比表面积、3 ~ 20 nm的宽孔径分布、良好的化学稳定性和优异的亲水性。在MOFs中引入分级孔不仅有利于磷酸肽进入内部金属亲和位点，降低其传质阻力，而且增加了金属亲和作用的暴露位点和Zr、Ti元素的结合能。得益于这些优点，THZr-MOFs对标准磷酸肽显示出高吸附容量（79.8 μg mg−1）。MALDI-TOF MS还获得了低检测限（0.05 fmol μL−1）和高富集选择性（β-酪蛋白/BSA摩尔比为1：5000）。THZr-MOFs应用于分析复杂样品，包括脱脂牛奶，人血清和HeLa细胞裂解物。总共，1432磷酸肽来自762磷蛋白被确定从人类HeLa细胞lysage.Graphical abstractionSchematic表示的应用程序的Ti 4 +-固定的层次多孔锆有机框架（表示为THZr-MOFs）在高效率和选择性富集低丰度磷酸肽从人HeLa细胞lysage. Graphical消化。
Ti4+-immobilized hierarchically porous zirconium-organic frameworks (denoted as THZr-MOFs) was prepared for phosphopeptide enrichment. The THZr-MOFs showed high specific surface area of 185.28 m2g−1, wide pore-size distribution of 3 ~ 20 nm, good chemical stability and excellent hydrophilicity. Introduction of hierarchical pores in MOFs not only facilitated the accessibility of phosphopeptides to the internal metal affinity sites and reduce their mass transfer resistance, but also increased the exposure sites of metal affinity interaction and binding energies of Zr and Ti elements. Benefited from these advantages, the THZr-MOFs showed high adsorption capacity (79.8 μg mg−1) towards standard phosphopeptide. A low detection limit (0.05 fmol μL−1) and high enrichment selectivity (β-casein/BSA with a molar ratio of 1:5000) were also obtained by MALDI-TOF MS. The THZr-MOFs were applied to analyze complex samples including nonfat milk, human serum, and HeLa cell lysate. In total, 1432 phosphopeptides derived from 762 phosphoproteins were identified from human HeLa cell lysate.Graphical abstractSchematic representation of the application of Ti4+-immobilized hierarchically porous zirconium-organic frameworks (denoted as THZr-MOFs) in high-efficiency and selective enrichment of low-abundance phosphopeptides from the tryptic digest of human HeLa cell lysate.