Targeting Plant Ethylene Responses by Controlling Essential Protein-Protein Interactions in the Ethylene Pathway
Targeting Plant Ethylene Responses by Controlling Essential Protein-Protein Interactions in the Ethylene Pathway
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DOI：
10.1016/j.molp.2015.03.014
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发表时间：
2015-08-03
期刊：
影响因子：
27.5
通讯作者：
Groth, Georg
中科院分区：
文献类型：
--
作者：
Bisson, Melanie M. A.;Groth, Georg
气态植物激素乙烯在植物的整个生命周期中调节许多具有高度农艺相关性的过程。乙烯信号传导的核心元件是内质网 (ER) 定位的膜蛋白 ETHYLENE INSENSITIVE2 (EIN2)。最近的研究表明，响应乙烯，EIN2 的膜外 C 末端被蛋白水解加工并从 ER 易位到细胞核。在此，我们报道了介导 EIN2 C 末端核输入的保守核定位信号 (NLS) 为与乙烯受体 ETHYLENE RESPONSE1 (ETR1) 形成复合物提供了重要的结构域。缺乏 NLS 结构域的 EIN2 显示出与受体的亲和力大大降低。 EIN2 和 ETR1 的相互作用也被 NLS 基序的合成肽阻断。相应的肽显着降低植物中的乙烯反应。我们的结果揭示了干扰植物乙烯信号转导和乙烯反应的抑制剂的新机制和类型。我们对 EIN2-ETR1 复合物的研究表明，乙烯信号通路中必需的蛋白质-蛋白质相互作用的破坏有可能指导现代农业和园艺创新乙烯拮抗剂的开发。
The gaseous plant hormone ethylene regulates many processes of high agronomic relevance throughout the life span of plants. A central element in ethylene signaling is the endoplasmic reticulum (ER)-localized membrane protein ETHYLENE INSENSITIVE2 (EIN2). Recent studies indicate that in response to ethylene, the extra-membranous C-terminal end of EIN2 is proteolytically processed and translocated from the ER to the nucleus. Here, we report that the conserved nuclear localization signal (NLS) mediating nuclear import of the EIN2 C-terminus provides an important domain for complex formation with ethylene receptor ETHYLENE RESPONSE1 (ETR1). EIN2 lacking the NLS domain shows strongly reduced affinity for the receptor. Interaction of EIN2 and ETR1 is also blocked by a synthetic peptide of the NLS motif. The corresponding peptide substantially reduces ethylene responses in planta. Our results uncover a novel mechanism and type of inhibitor interfering with ethylene signal transduction and ethylene responses in plants. Disruption of essential protein-protein interactions in the ethylene signaling pathway as shown in our study for the EIN2-ETR1 complex has the potential to guide the development of innovative ethylene antagonists for modern agriculture and horticulture.