Correlation of anisotropic cell behaviors with topographic aspect ratio
Correlation of anisotropic cell behaviors with topographic aspect ratio
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DOI：
10.1016/j.biomaterials.2008.11.041
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发表时间：
2009-03-01
期刊：
影响因子：
14
通讯作者：
Hu, W.
中科院分区：
文献类型：
--
作者：
Crouch, Adam S.;Miller, D.;Hu, W.
在这项研究中，我们使用纳米印迹在块状聚苯乙烯平板中创建了一系列微米和纳米级的栅格，或它们的组合，以研究人皮肤成纤维细胞相对于栅格的纵横比(深度/宽度)的各向异性细胞行为。聚苯乙烯格栅的深度和宽度分别对细胞取向和伸长率显示出强烈的影响，这在定性上与其他研究的结果相似。然而，这些个体参数与不同研究的一致定量比较因已测试的宽度和深度组合的多样性而变得复杂。相反，栅格的纵横比作为对栅格形貌的统一描述，是解释细胞形态的地形依赖性的更一致的参数。细胞取向和延伸率都随着长径比的增加而增加，即使是浅栅格(长宽比接近0.05)也足以诱导80%的细胞取向。尽管细胞类型和表面结构不同，其他小组最近发布的重新绘制数据与纵横比的关系也显示出类似的依赖关系。这一一致性表明，纵横比是表征细胞行为的一个通用因素。用接触导引理论解释了晶胞伸长和取向与地形纵横比的关系。该模型为与电池连接的设备和材料提供了简单和灵活的几何设计。爱思唯尔有限公司出版。
In this Study, we have used nanoimprinting to create a range of micro- and nanoscale gratings, or their combination, in bulk polystyrene plates to investigate anisotropic cell behaviors of human dermal fibroblasts with respect to the aspect ratio (depth/width) of gratings. The depth and width of the polystyrene gratings both show strong effects individually on cell alignment and elongation that are qualitatively similar to the results of other studies. However, consistent quantitative comparison of these individual parameters with different Studies is complicated by the diversity of combinations of width and depth that have been tested. Instead, the aspect ratio of the gratings as a unified description of grating topography is a more consistent parameter to interpret topographic dependence of cell morphology. Both cell alignment and elongation increase with increasing aspect ratio, and even a shallow grating (aspect ratio of similar to 0.05) is sufficient to induce 80% cell alignment. Re-plotting data recently published by other groups vs. aspect ratio shows a similar dependence, despite differences in cell types and surface Structures. This consistency indicates that aspect ratio is a general factor to characterize cell behaviors. The relationship of cell elongation and alignment with topographic aspect ratio is interpreted in terms of the theory of contact guidance. This model provides simplicity and flexibility in geometry design for devices and materials that interface with cells. Published by Elsevier Ltd.