CD203c expression on human basophils is associated with asthma exacerbation
CD203c expression on human basophils is associated with asthma exacerbation
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DOI：
10.1016/j.jaci.2009.10.074
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发表时间：
2010-02-01
影响因子：
14.2
通讯作者：
Akiyama, Kazuo
中科院分区：
文献类型：
--
作者：
Ono, Emiko;Taniguchi, Masami;Akiyama, Kazuo
背景资料：CD 203 c是一种嗜碱性粒细胞表面标志物，用于诊断和监测各种过敏性疾病，但其与哮喘的关系尚不清楚。目的：我们确定CD 203 c表达水平是否与哮喘稳定和加重有关。方法：我们采用流式细胞术比较哮喘稳定或加重患者和健康受试者嗜碱性粒细胞表面标志物的自发表达水平。这些表达水平的纵向变化后，嗜碱性粒细胞刺激IgE依赖性或IgE非依赖性的机制，并与患者的哮喘status.Results：CD 203 c的自发表达水平显着高于哮喘急性发作患者的嗜碱性粒细胞比稳定的哮喘或健康受试者。相反，在3组中未观察到CD 63或CD 69的自发表达水平的差异。在哮喘急性发作期间，抗IgE诱导的CD 203 c表达在嗜碱性粒细胞中显著增加（P=.005）。低浓度的屋尘螨或IL-3在哮喘急性发作期间诱导的CD 203 c表达水平高于临床改善期间;因此，这些抗原诱导的CD 203 c表达与哮喘控制相关。在临床症状改善的患者中，CD 203 c的自发表达水平与FEV（1）的百分比预测值之间存在相关性（r = -0.761; P = 0.022）。结论：哮喘急性发作时伴有嗜碱性粒细胞CD 203 c表达增加，缓解期CD 203 c表达显著降低。因此，CD 203 c的嗜碱性粒细胞表达水平可用于监测哮喘患者。（J Allergy Clin Immunol 2010;125：483-9.）
Background: CD203c is a basophil cell surface marker used to diagnose and monitor various allergic diseases, but its relationship to asthma is not clear.Objective: We determined whether CD203c expression levels are associated with stable and exacerbated asthma.Methods: We used flow cytometry to compare spontaneous expression levels of surface markers on basophils from patients with stable or exacerbated asthma and from healthy subjects. Longitudinal changes in these expression levels were measured after basophil stimulation by IgE-dependent or IgE-independent mechanisms and compared with patients' asthma status.Results: Spontaneous expression levels of CD203c were significantly higher on basophils from patients with asthma exacerbation than patients with stable asthma or healthy subjects. In contrast, no differences in spontaneous expression levels of CD63 or CD69 were observed among the 3 groups. Anti-IgE-induced expression of CD203c significantly increased in basophils during asthma exacerbation (P=.005). Low concentrations of Dermatophagoides pteronyssinus or IL-3 induced higher expression levels of CD203c during asthma exacerbation than during clinical improvement; induction of CD203c expression by these antigens therefore correlates with asthma control. In the patients with clinical improvement, there was a correlation between spontaneous CD203c expression levels and the percent predicted values of FEV(1) (r = -0.761; P = .022).Conclusion: Asthma exacerbation was accompanied by increased expression of CD203c on basophils that decreased significantly during remission. Basophil expression levels of CD203c might therefore be used to monitor asthma in patients. (J Allergy Clin Immunol 2010;125:483-9.)