Effects of Modified Simiao Decoction on IL-1β and TNFα Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation
Effects of Modified Simiao Decoction on IL-1β and TNFα Secretion in Monocytic THP-1 Cells with Monosodium Urate Crystals-Induced Inflammation
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DOI：
10.1155/2014/406816
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Dong, Hui
中科院分区：
文献类型：
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作者：
Liu, Ya-Fei;Tu, Sheng-Hao;Dong, Hui
四妙丸是一种含有四种草药的中药配方，多年来一直用于治疗痛风性关节炎。本研究的目的是探讨加味四妙汤 (MSD) 对尿酸钠 (MSU) 晶体诱导炎症的单核 THP-1 细胞 IL-1 β 和 TNF α 分泌的影响。通过佛波醇12-肉豆蔻酸酯13-乙酸酯(PMA)和MSU晶体的刺激，成功建立了THP-1细胞中MSU晶体诱导的炎症模型。然后，将MSD衍生的血清或从大鼠中提取的对照血清给予不同的治疗组。观察MSU晶体和THP-1细胞的形态。通过ELISA测定THP-1细胞上清液中IL-1β和TNFα蛋白的表达。我们的数据表明，MSU 晶体诱导 IL-1 β 和 TNF α 的时间依赖性增加。此外，MSD 显着降低了 MSU 晶体诱导炎症的 THP-1 细胞中 IL1 β 的释放。这些结果表明，MSD 在治疗 THP-1 细胞中 MSU 晶体诱导的炎症方面很有前景。 MSD 可作为抗 IL-1 药物治疗痛风。其潜在机制可能与NALP3炎症小体有关，需要在未来的研究中进行验证。
Simiao pill, a Chinese herbal formula containing four herbs, has been used in the treatment of gouty arthritis for many years. The aim of this study was to explore the effects of modified Simiao decoction (MSD) on IL-1 beta and TNF alpha secretion in monocytic THP-1 cells with monosodium urate (MSU) crystals-induced inflammation. The MSU crystals-induced inflammation model in THP-1 cells was successfully established by the stimulation of phorbol 12-myristate 13-acetate (PMA) and MSU crystals. Then, the MSD-derived serum or control serum extracted from rat was administered to different treatment groups. The morphology of MSU crystals and THP-1 cells was observed. IL-1 beta and TNF alpha protein expression in supernatant of THP-1 cells were determined by ELISA. Our data demonstrated that MSU crystals induced time-dependent increase of IL-1 beta and TNF alpha Moreover, MSD significantly decreased IL1 beta release in THP-1 cells with MSU crystals-induced inflammation. These results suggest that MSD is promising in the treatment of MSU crystals-induced inflammation in THP-1 cells. MSD may act as an anti-IL-1 agent in treating gout. The underlying mechanism may be related to NALP3 inflammasome which needs to be validated in future studies.