Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
复制标题
DOI：
10.1371/journal.pone.0048508
复制
发表时间：
2012-11-14
期刊：
影响因子：
3.7
通讯作者：
Pessler, Frank
中科院分区：
文献类型：
--
作者：
Akmatov, Manas K.;Gatzemeier, Anja;Pessler, Frank
背景：在症状发作期间及时收集诊断性生物样本的需求是急性呼吸道感染（ARI）流行病学大规模研究的主要障碍。这可以通过让参与者自己收集鼻拭子来避免。我们比较了自己和工作人员采集的拭子在拭子质量和病毒性呼吸道病原体检测方面的差异。方法/主要发现：我们在2010/2011 ARI季节（12月至3月）对我们机构的员工进行了一项前瞻性研究。每周向参与者（n = 84）发送电子邮件，提醒他们在出现新症状时来研究中心。参与者自己从一个鼻孔收集鼻前拭子，训练有素的研究人员从另一个鼻孔收集拭子。在接下来的一天，参与者自己收集了另外两个拭子（每个鼻孔一个）。测定拭子中人-肌动蛋白DNA浓度作为质量控制。采用多重RT-PCR检测呼吸道病毒病原体（Seeplex RV15试剂盒，Seegene, Eschborn，德国）。在84名参与者中，56名（67%）报告了至少一次ARI发作，18名参与者两次，1名参与者三次。自我擦拭被参与者高度接受。自拭子中-肌动蛋白DNA的含量高于工作人员收集的拭子（p = 0.008）。第1天收集的自拭子中β -肌动蛋白浓度低于随后一天收集的自拭子（p < 0.0001）。在31%（23/75）的工作人员和35%（26/75）的自采拭子中检测到呼吸道病毒病原体（p = 0.36）。两种方法中，最常见的病原体是人鼻病毒A/B/C（12/75拭子，16%）和人冠状病毒OC43（4/75拭子，5%）。自拭子与工作人员拭子在病原菌检出率方面几乎完全一致（一致性= 93%,kappa = 0.85, p < 0.0001）。结论/意义：在该人群中，自拭鼻法鉴定病毒性ARI病原体在接受度和病原体检测方面与工作人员拭鼻法相当。
Background: The need for the timely collection of diagnostic biosamples during symptomatic episodes represents a major obstacle to large-scale studies on acute respiratory infection (ARI) epidemiology. This may be circumvented by having the participants collect their own nasal swabs. We compared self- and staff-collected swabs in terms of swabbing quality and detection of viral respiratory pathogens.Methodology/Principal Findings: We conducted a prospective study among employees of our institution during the ARI season 2010/2011 (December-March). Weekly emails were sent to the participants (n = 84), reminding them to come to the study center in case of new symptoms. The participants self-collected an anterior nasal swab from one nostril, and trained study personnel collected one from the other nostril. The participants self-collected another two swabs (one from each nostril) on a subsequent day. Human beta-actin DNA concentration was determined in the swabs as a quality control. Viral respiratory pathogens were detected by multiplex RT-PCR (Seeplex RV15 kit, Seegene, Eschborn, Germany). Of 84 participants, 56 (67%) reported at least one ARI episode, 18 participants two, and one participant three. Self-swabbing was highly accepted by the participants. The amount of beta-actin DNA per swab was higher in the self-than in the staff-collected swabs (p = 0.008). beta-actin concentration was lower in the self-swabs collected on day 1 than in those collected on a subsequent day (p < 0.0001). A respiratory viral pathogen was detected in 31% (23/75) of staff- and in 35% (26/75) of self-collected swabs (p = 0.36). With both approaches, the most frequently identified pathogens were human rhinoviruses A/B/C (12/75 swabs, 16%) and human coronavirus OC43 (4/75 swabs, 5%). There was almost perfect agreement between self- and staff-collected swabs in terms of pathogen detection (agreement = 93%, kappa = 0.85, p < 0.0001).Conclusions/Significance: Nasal self-swabbing for identification of viral ARI pathogens proved to be equivalent to staff-swabbing in this population in terms of acceptance and pathogen detection.