RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations.
RNA sequencing-based analysis of the spleen transcriptome following infectious bronchitis virus infection of chickens selected for different mannose-binding lectin serum concentrations.
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DOI：
10.1186/s12864-016-2403-1
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发表时间：
2016-01-27
期刊：
影响因子：
4.4
通讯作者：
Dalgaard TS
中科院分区：
文献类型：
--
作者：
Hamzić E;Kjærup RB;Mach N;Minozzi G;Strozzi F;Gualdi V;Williams JL;Chen J;Wattrang E;Buitenhuis B;Juul-Madsen HR;Dalgaard TS
鸡传染性支气管炎是由鸡传染性支气管炎病毒（IBV）引起的一种上呼吸道高度接触性传染病。了解IBV感染的先天性和适应性免疫应答之间相互作用的分子机制是进一步改进控制IB策略的关键因素。为此，选择了两个高（L10 H系）和低（L10 L系）血清甘露糖结合凝集素（MBL）浓度的鸡系进行了研究。总共使用来自每个品系的32只鸡。来自每个系的16只鸡感染IBV，16只未感染。在感染后1周和3周，将来自每个品系的8只未感染和感染的鸟安乐死。对来自所有64只鸟的脾样品进行RNA测序，并进行差异基因表达分析以进行四次比较：分别在第1周和第3周，未感染鸟的L10 L系与L10 H系，以及感染鸟的相同方式。使用基因本体（GO）免疫系统过程术语对原鸡进行功能分析。比较未感染的L10 H和L10 L鸟，我们确定了1698和1424差异表达（DE）基因在第1周和第3周，分别。对于IBV感染的鸟，1934和866 DE基因之间的两个线在第1周和第3周，分别确定。从两个品系之间的未感染禽类的比较中出现的两个最丰富的GO术语分别是第1周和第3周的“参与免疫应答的淋巴细胞活化”和“参与免疫应答的免疫球蛋白基因的体细胞重组”。当比较两个品系之间的IBV感染的禽类时，最丰富的GO术语分别是第1周和第3周的“α-β T细胞活化”和“白细胞活化的正调节”。来自这两个系的健康鸟类在适应性和先天免疫相关基因子集的表达谱上显示出显着差异，而来自这两个系的IBV感染鸟类的比较显示参与T细胞激活和增殖的免疫相关基因的表达差异。观察到的两个系之间的转录组差异表明，MBL的选择影响了先天免疫和适应性免疫。本文的在线版本（doi：10.1186/s12864-016-2403-1）包含补充材料，可供授权用户使用。
Avian infectious bronchitis is a highly contagious disease of the upper-respiratory tract caused by infectious bronchitis virus (IBV). Understanding the molecular mechanisms involved in the interaction between innate and adaptive immune responses to IBV infection is a crucial element for further improvements in strategies to control IB. To this end, two chicken lines, selected for high (L10H line) and low (L10L line) serum concentration of mannose-binding lectin (MBL) were studied. In total, 32 birds from each line were used. Sixteen birds from each line were infected with IBV and sixteen were left uninfected. Eight uninfected and infected birds from each line were euthanized at 1 and 3 weeks post infection. RNA sequencing was performed on spleen samples from all 64 birds and differential gene expression analysis was performed for four comparisons: L10L line versus L10H line for uninfected birds at weeks 1 and 3, respectively, and in the same way for infected birds. Functional analysis was performed using Gene Ontology (GO) Immune System Process terms specific for Gallus gallus. Comparing uninfected L10H and L10L birds, we identified 1698 and 1424 differentially expressed (DE) genes at weeks 1 and 3, respectively. For the IBV-infected birds, 1934 and 866 DE genes were identified between the two lines at weeks 1 and 3, respectively. The two most enriched GO terms emerging from the comparison of uninfected birds between the two lines were “Lymphocyte activation involved in immune response” and “Somatic recombination of immunoglobulin genes involved in immune response” at weeks 1 and 3, respectively. When comparing IBV-infected birds between the two lines, the most enriched GO terms were “Alpha-beta T cell activation” and “Positive regulation of leukocyte activation” at weeks 1 and 3, respectively. Healthy birds from the two lines showed significant differences in expression profiles for subsets of adaptive and innate immunity-related genes, whereas comparison of the IBV-infected birds from the two lines showed differences in expression of immunity-related genes involved in T cell activation and proliferation. The observed transcriptome differences between the two lines indicate that selection for MBL had influenced innate as well as adaptive immunity. The online version of this article (doi:10.1186/s12864-016-2403-1) contains supplementary material, which is available to authorized users.