Microbial community dynamics in a larval aquaculture system of the tropical rock lobster, Panulirus ornatus
Microbial community dynamics in a larval aquaculture system of the tropical rock lobster, Panulirus ornatus
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DOI：
10.1016/j.aquaculture.2004.08.047
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发表时间：
2004-12-20
期刊：
影响因子：
4.5
通讯作者：
Hall, M
中科院分区：
文献类型：
--
作者：
Bourne, DG;Young, N;Hall, M
到目前为止，热带岩龙虾(Panulirus Ornatus)的商业化规模养殖一直不成功，尝试的特点是早期孵化场饲养的幼体周期性地大量死亡。在这里，我们调查了影响早期叶状瘤的微生物和组织病理学因素。组织病理学证实了叶状瘤的微生物感染，包括肝胰腺内细菌的增殖以及附属物和角质层与丝状细菌和定植原生动物的严重外部污垢。扫描电子显微镜证实了至少两种形态的丝状细菌和杆状细菌的污染。表明了一个多样化的附生生物群落。对标准幼虫饲养环境的水柱、水箱生物膜和整个叶状瘤进行了基于培养和分子的微生物群落分析。细菌数量(以菌落形成单位[CFU]毫升(-1)衡量)和叶状瘤死亡率之间没有相关性。基于选择性培养的研究表明，弧菌科相关生物是微生物群落的优势部分，副溶血性弧菌是从所调查的每个环境中分离到的最常见的微生物。在基于培养的研究中观察到有限的微生物系统发育多样性，与基于分子的变性梯度凝胶电泳(DGGE)结果相比较。水、生物膜和叶状藻环境的DGGE图谱不同。表明独特的微生物生态位维持着不同的微生物种群。这些微生物种群似乎是动态的，因为DGGE图谱在实验过程中在每个环境中都发生了变化。从DGGE图谱中检索到的弧菌附属序列很少，突出了两种评估幼虫饲养系统内微生物多样性的方法之间的差异。(C)2004爱思唯尔B.V.保留所有权利。
Commercial scale rearing of the tropical rock lobster (Panulirus ornatus) has been unsuccessful to date, with attempts characterised by periodic mass mortalities of early stage hatchery-reared larvae. Here, we investigate the microbiological and histopathological factors affecting early stage phyllosomas. Histopathology identified microbial infestation of phyllosomas, including proliferation of bacteria in the hepatopancreas and heavy external fouling of appendages and cuticle with filamentous bacteria and sessile protozoa. Scanning electron microscopy confirmed fouling by filamentous bacteria of at least two morphological types and also fouling by rod-shaped bacteria. indicative of a diverse epibiont community. Culture- and molecular-based microbial community analysis was performed oil the water column, tank biofilm and whole phyllosomas of standard larval-rearing environments. No correlation between bacterial numbers (measured as colony-forming units [CFU] mL(-1)) and phyllosoma mortalities was established. Culture-based studies using selective media indicated that Vibrionaceae-related organisms were a dominant part of the microbial community and Vibrio parahaemolyticus was the most commonly isolated organism from each environment investigated. Limited microbial phylogenetic diversity was observed for culture-based studies when compared against molecular-based denaturing gradient gel electrophoresis (DGGE) results. DGGE profiles of water, biofilm and phyllosoma environments differed. indicative of unique microbial niches sustaining different microbial populations. These microbial populations appeared dynamic since DGGE profiles changed within each environment over the course of the experiment. Few Vibrio-affiliated sequences were retrieved from DGGE profiles highlighting differences between the two methods for assessing microbial diversity within the larval-rearing system. (C) 2004 Elsevier B.V. All rights reserved.