Chemical Evolution of a Bacterial Proteome.
Chemical Evolution of a Bacterial Proteome.
复制标题
DOI：
10.1002/anie.201502868
复制
发表时间：
2015-08-17
期刊：
影响因子：
--
通讯作者：
Budisa N
中科院分区：
文献类型：
--
作者：
Hoesl MG;Oehm S;Durkin P;Darmon E;Peil L;Aerni HR;Rappsilber J;Rinehart J;Leach D;Söll D;Budisa N
我们已经通过进化能够在合成的非规范氨基酸L-β-（噻吩并[3，2-B]吡咯基）-丙氨酸（[3，2]Tpa）上生长的培养物作为规范氨基酸L-色氨酸（Trp）的唯一替代物来改变大肠杆菌遗传密码的氨基酸组。在确定的合成培养基中进行的长期培养实验导致细胞的进化，这些细胞能够在其蛋白质组中响应于E.大肠杆菌W3110基因组。这些进化的细菌具有新的天然氨基酸组成，能够在完全不存在Trp的情况下稳健生长。我们的实验结果说明了一种方法的替代生化积木的合成细胞的进化。对于合成细菌：在合成培养基中的进化实验导致响应于细菌大肠杆菌W3110中的20，899个TGG密码子的定量色氨酸至L-β-（噻吩并[3，2-B]吡咯基）-丙氨酸取代。进化的细菌能够在完全不存在Trp的情况下稳健生长，而对细胞存活没有显著的不利影响。
We have changed the amino acid set of the genetic code of Escherichia coli by evolving cultures capable of growing on the synthetic non-canonical amino acid L-β-(thieno[3,2-b]pyrrolyl)-alanine ([3,2]Tpa) as a sole surrogate for the canonical amino acid L-tryptophan (Trp). A long-term cultivation experiment in defined synthetic media resulted in the evolution of cells capable of surviving Trp → [3,2] Tpa substitutions in their proteomes in response to the 20,899 TGG codons of the E. coli W3110 genome. These evolved bacteria with new-to-nature amino acid composition are capable of robust growth in the complete absence of Trp. Our experimental results illustrate an approach for the evolution of synthetic cells with alternative biochemical building blocks. Towards synthetic bacteria: An evolution experiment in synthetic medium led to quantitative Tryptophan to L-β-(thieno[3,2-b]pyrrolyl)-alanine substitution in response to 20,899 TGG codons in bacterium Escherichia coli W3110. The evolved bacteria were capable of robust growth in the complete absence of Trp without significant adverse effects on the cellular survival.