Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates
Yeast Cbk1 and Mob2 activate daughter-specific genetic programs to induce asymmetric cell fates
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DOI：
10.1016/s0092-8674(01)00596-7
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发表时间：
2001-12-14
期刊：
影响因子：
64.5
通讯作者：
Brent, R
中科院分区：
文献类型：
--
作者：
Colman-Lerner, A;Chin, TE;Brent, R
在酿酒酵母中，母亲和女儿有不同的命运。我们发现，Cbk 1激酶和它的相互作用蛋白Mob 2调节这种不对称性诱导的女儿特定的遗传程序。子代特异性表达是由于Cbk 1/Mob 2依赖性激活和Ace 2转录因子定位于子代细胞核。活性Ace 2异位定位于母体细胞核足以激活母体中的子体特异性基因。在测试条件下，八个基因是子体特异性的，而两个仅在饱和培养物中是子体特异性的。一些子体特异性基因产物通过降解细胞壁而促进细胞分离。这些实验定义了特定于女儿的基因表达程序，并描述了这些程序是如何控制的。
In Saccharomyces cerevisiae, mothers and daughters have distinct fates. We show that Cbk1 kinase and its interacting protein Mob2 regulate this asymmetry by inducing daughter-specific genetic programs. Daughter-specific expression is due to Cbk1/Mob2-dependent activation and localization of the Ace2 transcription factor to the daughter nucleus. Ectopic localization of active Ace2 to mother nuclei is sufficient to activate daughter-specific genes in mothers. Eight genes are daughter-specific under the tested conditions, while two are daughter-specific only in saturated cultures. Some daughter-specific gene products contribute to cell separation by degrading the cell wall. These experiments define programs of gene expression specific to daughters and describe how those programs are controlled.