Weak before strong: dissociating synaptic tagging and plasticity-factor accounts of late-LTP
Weak before strong: dissociating synaptic tagging and plasticity-factor accounts of late-LTP
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DOI：
10.1016/s0028-3908(98)00040-9
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发表时间：
1998-04-01
影响因子：
4.7
通讯作者：
Morris, RGM
中科院分区：
文献类型：
--
作者：
Frey, U;Morris, RGM
实验是用体外海马片进行的，以比较两种可能实现输入特异性蛋白质合成依赖的长时程增强(LTP)的机制。突触标签假说(Frey and Morris，1997)预测，弱破伤风后早期LTP的表达可以通过随后另一条单独的路径的强烈破伤风稳定到晚期LTP，前提是两次破伤风发作之间的间隔在假定的突触标签的衰减时间过程内。另一种可塑性因素假说要求强破伤风应始终先于弱破伤风，才能使早期LTP稳定。我们的结果表明，在强破伤风之前，在强破伤风之前5分钟或1小时对路径S2进行弱破伤风确实会导致路径S2的LTP延迟。稳定作用较弱或不发生在2和4小时的间隔。这种稳定作用被证明依赖于在S1强烈破伤风期间的蛋白质合成。这些发现支持了突触标记假说的一个关键预测，并暗示了突触标记对皮质可塑性的功能作用。(C)1998爱思唯尔科学有限公司。保留所有权利。
Experiments were conducted using hippocampal slices in vitro to compare two accounts of the mechanisms by which input-specific protein synthesis-dependent long-term potentiation (late-LTP) may be realised. The synaptic tag hypothesis (Frey and Morris, 1997) predicts that the expression of early-LTP following a weak tetanus can be stabilised into late-LTP by subsequent strong tetanisation of a separate pathway, provided the interval between the two tetanisation episodes is within the decay time-course of a putative synaptic tag. An alternative plasticity-factors hypothesis requires that strong tetanisation should always precede weak tetanisation for stabilisation of early-LTP to occur. Our results indicate that weak tetanisation of pathway S2 at intervals of 5 min or 1 h prior to strong tetanisation on pathway S1 does result in late-LTP on pathway S2. Stabilisation was weaker or did not occur at intervals of 2 and 4 h. This stabilisation effect was shown to depend on protein synthesis during the strong tetanisation of S1. These findings uphold a key prediction of the synaptic tag hypothesis and have implications for the functional role of synaptic tagging for cortical plasticity. (C) 1998 Elsevier Science Ltd. All rights reserved.