Synthesis and Physicochemical Characterization of Biodegradable and pH-Responsive Hydrogels Based on Polyphosphoester for Protein Delivery
Synthesis and Physicochemical Characterization of Biodegradable and pH-Responsive Hydrogels Based on Polyphosphoester for Protein Delivery
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用于蛋白质递送的基于聚磷酸酯的可生物降解和 pH 响应性水凝胶的合成和理化表征
DOI：
10.1002/pola.23959
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发表时间：
2010-05-01
影响因子：
--
通讯作者：
Zhu, Xiulin
中科院分区：
文献类型：
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作者：
He, Jinlin;Ni, Peihong;Zhu, Xiulin
在这项工作中，提出了一系列基于聚磷酸酯和聚丙烯酸的可生物降解和ph响应的水凝胶。以HEMA为引发剂，Sn(Oct)(2)为催化剂，以2-乙氧基-2-氧-1,3,2-二氧磷烷为环单体，通过开环聚合，将羟基转化为乙烯基，合成了一种新型可生物降解的大型交联剂α -甲基丙烯酰氧乙基- omega-丙烯酰聚（乙基乙烯磷酸）（HEMA- peop -ac）。然后将该高分子单体与丙烯酸共聚制备水凝胶，并对其溶胀/溶胀和降解行为进行了研究。结果表明，交联密度和介质pH值对水凝胶的溶胀率和降解率均有较大影响。研究了这些水凝胶的流变性能，发现其储存模量（G′）与交联密度有明显的关系。MTT和“活/死”实验表明，这些水凝胶与成纤维细胞相容，即使在高浓度下也没有表现出明显的细胞毒性。此外，还研究了这些水凝胶的体外牛血清白蛋白释放情况，发现释放曲线表现为爆发效应，然后是连续释放期，并且释放速度与水凝胶的交联密度成反比。(C) 2010 Wiley期刊公司[J]高分子学报，A辑：高分子化学48 (1):19930,2010
In this work, a series of biodegradable and pH-responsive hydrogels based on polyphosphoester and poly (acrylic acid) are presented. A novel biodegradable macro-crosslinker alpha-methacryloyloxyethyl omega-acryloyl poly(ethyl ethylene phosphate) (HEMA-PEOP-Ac) was synthesized by first ring-opening polymerization of the cyclic monomer 2-ethoxy-2-oxo-1,3,2-dioxaphospholane using HEMA as the initiator and Sn(Oct)(2) as catalyst, and subsequent conversion of hydroxyl into vinyl group. The hydrogels were then fabricated by the copolymerization of the macromonomer with acrylic acid, and their swelling/deswelling and degradation behaviors were investigated. The results demonstrated that the crosslinking density and pH values of media strongly influenced both the swelling ratio and the degradation rate of the hydrogels. The rheological properties of these hydrogels were also studied from which the storage modulus (G') showed clear dependence on the crosslinking density. MTT and "live/dead" assay showed that these hydrogels were compatible to fibroblast cells, not exhibiting apparent cytotoxicity even at high concentrations. Moreover, in vitro bovine serum albumin release from these hydrogels was also investigated, and it could be found that the release profiles showed a burst effect followed by a continuous release phase, and the release rate was inversely proportional to the crosslinking density of hydrogels. (C) 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 1919 1930, 2010