CHEMOTAXIS OF MONOCYTES AND NEUTROPHILS TO PLATELET-DERIVED GROWTH-FACTOR
CHEMOTAXIS OF MONOCYTES AND NEUTROPHILS TO PLATELET-DERIVED GROWTH-FACTOR
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DOI：
10.1172/jci110509
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发表时间：
1982-01-01
影响因子：
15.9
通讯作者：
HUANG, JS
中科院分区：
文献类型：
--
作者：
DEUEL, TF;SENIOR, RM;HUANG, JS
[人]血小板衍生生长因子(PDGF)对单核细胞和中性粒细胞具有趋化作用。单核细胞对PDGF的最大趋化性与C5a完全相同，并且发生在.simeq。20 ng/ml(.simeq.0.7海里)。最大中性粒细胞趋化性为.simeq。C5a的60%，但发生在.simeq。Ng/ml(.simeq.下午32：00)。PDGF的趋化活性可被PDGF的特异性抗血清和与细胞表面结合的竞争性PDGF抑制剂鱼精蛋白所阻断。与PDGF相比，表皮生长因子在0.5-100 ng/ml范围内对炎症细胞没有趋化活性。PDGF的高度趋化活性表明，除了作为平滑肌细胞和成纤维细胞的有丝分裂原外，PDGF可能还参与了将炎症细胞吸引到血小板释放部位的作用。
The [human] platelet-derived growth factor (PDGF) is chemotactic for monocytes and neutrophils. Maximum monocyte chemotaxis to PDGF is fully equal to that achieved with C5a and occurs at .simeq. 20 ng/ml (.simeq. 0.7 nM). Maximum neutrophil chemotaxis is .simeq. 60% that achieved with C5a but occurs at .simeq. ng/ml (.simeq. 32 pM). The chemotactic activity of PDGF is blocked by specific antisera to PDGF and by protamine sulfate, a competitive inhibitor of PDGF binding to cell surfaces. In contrast to PDGF, epidermal growth factor shows no chemotactic activity for inflammatory cells at 0.5-100 ng/ml. The high level of chemotactic activity of PDGF suggests that in addition to its role role as a mitogen for smooth muscle cells and fibroblasts, PDGF may be involved in attracting inflammatory cells to sites of platelet release.