IMMUNOLOGY, HEALTH, AND DISEASE Abnormal histopathology, fat percent and hepatic apolipoprotein A I and apolipoprotein B100 mRNA expression in fatty liver hemorrhagic syndrome and their improvement by soybean lecithin
IMMUNOLOGY, HEALTH, AND DISEASE Abnormal histopathology, fat percent and hepatic apolipoprotein A I and apolipoprotein B100 mRNA expression in fatty liver hemorrhagic syndrome and their improvement by soybean lecithin
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免疫学、健康和疾病 脂肪肝出血综合征中异常的组织病理学、脂肪百分比以及肝载脂蛋白 A I 和载脂蛋白 B-100 mRNA 表达及其大豆卵磷脂的改善
DOI：
10.3382/ps/pex163
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发表时间：
2017-10-01
期刊：
影响因子：
4.4
通讯作者：
Hu, Guoliang
中科院分区：
文献类型：
--
作者：
Song, Yalu;Ruan, Jiming;Hu, Guoliang
为探讨蛋鸡脂肪肝出血综合征（FLHS）的发病机制及大豆卵磷脂对FLHS的保护作用，选用健康300日龄海兰蛋鸡135只，随机分为对照组（1组）、患病组（2组）和保护组（3组）。每组45只，每组3个重复。3组分别饲喂对照饲粮、高能低蛋白（HELP）饲粮或在HELP饲粮中添加3%大豆卵磷脂代替玉米。计算肝脏脂肪含量。采用染色法检测肝脏组织病理变化，RT-PCR法检测肝脏载脂蛋白ina I （apoA I）和载脂蛋白B-100 (apoB(100)) mRNA表达水平。结果表明：第30、60天，第2组肝脏脂肪率显著高于第1、2组（P < 0.01）；第2组30、60天肝脏组织学显示不同程度的肝脏病变，第3组肝细胞结构正常，30、60天肝细胞出现轻度微泡脂肪变性。在整个实验过程中，肝脏中apoA I和apoB100的mRNA表达水平是变化的。第60 d时，2组apoA I表达量显著降低（P < 0.05）；第2组apoB100的表达量在d 30时略有升高，而在d 60时急剧下降。与1组相比，3组apoB100的表达量无显著差异（P < 0.05）。本研究表明，FLHS可引起蛋鸡肝脏apoA I和apoB100的病理改变和异常表达，而大豆卵磷脂可减轻这些异常表达。
To investigate the etiopathogenesis of fatty liver hemorrhagic syndrome (FLHS) and the protective effects of soybean lecithin against FLHS in laying hens, 135 healthy 300-day-old Hyline laying hens were randomly divided into groups: control (group 1), diseased (group 2), and protected (group 3). Each group contained 45 layers with 3 replicates. The birds in these 3 groups were fed a control diet, a high-energy/low-protein (HELP) diet or the HELP diet supplemented with 3% soybean lecithin instead of maize. The fat percent in the liver was calculated. Histopathological changes in the liver were determined by staining, and the mRNA expression levels of apolipoproteinA I (apoA I) and apolipoprotein B-100 (apoB(100)) in the liver were determined by RT-PCR. The results showed that the fat percent in the liver of group 2 was much higher (P < 0.01) than that of group 1 and group 2 on d 30 and 60. The histology of the liver in group 2 on d 30 and 60 displayed various degrees of liver lesions, while the hepatocytes showed a normal structure in group 3 with mild microvesicular steatosis in the liver cell on d 30 and 60. The mRNA expression levels of apoA I and apoB100 in the livers were variable throughout the experiment. The expression level of apoA I in group 2 significantly decreased on d 60 (P < 0.05); the expression level of apoB100 slightly increased on d 30 in group 2, while it sharply decreased on d 60. Compared to group 1, the expression level of apoB100 showed no significant difference in group 3 (P < 0.05). This study indicated that FLHS induced pathological changes and abnormal expression of apoA I and apoB100 in the livers of laying hens and that soybean lecithin alleviated these abnormal changes.