The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1).
The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1).
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虾线粒体 FoF1-ATP 酶抑制因子 1 (IF1)。
DOI：
10.1007/s10863-015-9621-0
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发表时间：
2015
影响因子：
3
通讯作者：
Chimeo C
中科院分区：
文献类型：
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作者：
Chimeo C
凡纳滨对虾暴露在海水溶解氧浓度的周期性变化中，必须以三磷酸腺苷为能源中和低氧的不利影响。在甲壳类动物中，线粒体FOF1-ATP合成酶对ATP的动态平衡起关键作用，并且普遍作为FOF1-ATPase发挥作用。到目前为止，尚不清楚这些海洋无脊椎动物是否配备了能够控制抑制ATP消耗的FOF1-ATPase的分子。在这项研究中，我们报道了线粒体FOF1-ATPase抑制因子1(IF1)在凡纳滨对虾转录组组织中普遍表达的两个变体：IF1_Lv1和IF1_Lv2。IF1_LV1基因全长550个碱基，编码104个氨基酸的蛋白质，其信使核糖核酸含量受低氧和复氧的影响。IF1_Lv2全长654个碱基，编码85个氨基酸的蛋白质。这两种蛋白都有69%的同源性，都包含一个保守的最小抑制序列(IATP域)，以及它们N端典型的无脊椎动物特有的富含G的区域。根据这一特征，本文讨论了IF1作为该海洋生物进化出的一种适应机制，以抑制FOF1-ATPase活性，避免ATP耗散而在氧分压发生变化的情况下茁壮成长。
The whiteleg shrimp speciesLitopenaeus vannameiis exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of theLitopenaeus vannameitranscriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension.