Micropipette and vascular occlusion pressures in isolated lungs of newborn lambs.
Micropipette and vascular occlusion pressures in isolated lungs of newborn lambs.
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新生羔羊离体肺中的微量移液器和血管闭塞压力。
DOI：
10.1152/jappl.1993.75.4.1854
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kaplowitz,MR
中科院分区：
文献类型：
--
作者：
Fike,CD;Gordon,JB;Kaplowitz,MR
我们比较了通过血管闭塞、双闭塞和微穿刺技术对 3 至 10 日龄羔羊的离体灌注肺进行的测量。在16个肺的灌注液中添加吲哚美辛后，我们使用微穿刺技术测量直径20至80微米的小动脉（Pa80）、直径20至50微米的小静脉（Pv20）和直径50至150微米的小静脉（Pv150）的压力。在这些相同的肺中使用血管闭塞和双闭塞技术来测量流入闭塞（Pao）、流出闭塞（Pvo）和双闭塞压力（Pdo）。在灌注液中未添加吲哚美辛的其他 14 个肺中，我们在肺泡缺氧之前和期间和/或在灌注液中添加罂粟碱之前和之后测量了 Pv20 和 Pvo。在吲哚美辛治疗的肺部，Pao大于Pa80，Pvo等于Pv20和Pv150，Pdo与Pa80、Pv20和Pv150相同。在未经吲哚美辛治疗的肺部，缺氧前和缺氧期间的 Pvo 均小于 Pv20，但在灌注液中添加罂粟碱后，Pvo 等于 Pv20。因此，在用吲哚美辛处理的新生羔羊的肺部中，Pao反映了> 80微米直径的动脉压力，Pdo反映了80微米直径的动脉和150微米直径的静脉之间的压力，而Pvo则与小至20微米直径和大至150微米直径的静脉压力相似。然而，在没有吲哚美辛的情况下，在缺氧之前和缺氧期间，Pvo 和 Pv20 之间都存在梯度，但在使用罂粟碱降低血管舒缩张力之后则没有。因此，Pv20 和 Pv150 中 Pvo 与微穿刺压力之间的相关性并不恒定，并且似乎与血管舒缩张力相关。
We compared measurements made by vascular occlusion, double occlusion, and micropuncture techniques in isolated perfused lungs of 3- to 10-day-old lambs. After adding indomethacin to the perfusate of 16 lungs, we used the micropuncture technique to measure pressures in 20- to 80-microns-diam arterioles (Pa80), 20- to 50-microns-diam venules (Pv20), and 50- to 150-microns-diam venules (Pv150). The vascular occlusion and double occlusion techniques were used in these same lungs to measure inflow occlusion (Pao), outflow occlusion (Pvo), and double occlusion pressures (Pdo). In 14 other lungs without indomethacin added to the perfusate, we measured Pv20 and Pvo before and during alveolar hypoxia and/or before and after the addition of papaverine to the perfusate. In indomethacin-treated lungs, Pao was greater than Pa80, Pvo equaled Pv20 and Pv150, and Pdo was the same as Pa80, Pv20, and Pv150. In lungs without indomethacin treatment, Pvo was less than Pv20 both before and during hypoxia but Pvo equaled Pv20 after papaverine was added to the perfusate. Thus, in indomethacin-treated lungs of newborn lambs, Pao reflected pressure in arteries > 80 microns diam, Pdo reflected pressure between 80-microns-diam arteries and 150-microns-diam veins, and Pvo was similar to pressure in veins both as small as 20 microns diam and as large as 150 microns diam. However, without indomethacin there was a gradient between Pvo and Pv20 both before and during hypoxia but not after vasomotor tone was reduced with papaverine. Thus, the correlation between Pvo and micropuncture pressure in Pv20 and Pv150 was not constant and appeared related to vasomotor tone.