Surface-modified multifunctional MIP nanoparticles.
Surface-modified multifunctional MIP nanoparticles.
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DOI：
10.1039/c3nr00354j
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发表时间：
2013-05-07
期刊：
影响因子：
6.7
通讯作者：
Piletsky S
中科院分区：
文献类型：
--
作者：
Moczko E;Poma A;Guerreiro A;Perez de Vargas Sansalvador I;Caygill S;Canfarotta F;Whitcombe MJ;Piletsky S
采用一种新的固相方法在固定模板上合成了核壳分子印迹聚合物纳米颗粒（MIP NPs）。在随后的衍生化/壳形成过程中，同样的固相也对高亲和结合位点起保护作用。该程序允许快速合成，控制分离和纯化高亲和力材料，每个生产周期只需2小时。这种方法的目的是在纳米尺度上合成均匀尺寸的印迹材料，这种材料可以很容易地与各种聚合物接枝，而不会影响它们的亲和力和特异性。为了演示目的，我们将抗三聚氰胺MIP NPs与引入以下表面特性的涂层接枝：高极性（PEG甲基丙烯酸酯）；电活性（乙烯基二茂铁）；荧光（丙烯酸伊红）；巯基（季戊四醇四基（3-巯基丙酸））。该方法具有广泛的适用性，可用于生产多功能印迹纳米颗粒，在生物传感器、诊断和生物医学领域具有进一步应用的潜力，并可作为天然受体的替代品。
The synthesis of core-shell molecularly imprinted polymer nanoparticles (MIP NPs) has been performed using a novel solid-phase approach on immobilised templates. The same solid phase also acts as protective functionality for high affinity binding sites during subsequent derivatisation/shell formation. This procedure allows for the rapid synthesis, controlled separation and purification of high-affinity materials, with each production cycle taking just 2 hours. The aim of this approach is to synthesise uniformly-sized imprinted materials at the nanoscale which can be readily grafted with various polymers without affecting their affinity and specificity. For demonstration purposes we grafted anti-melamine MIP NPs with coatings which introduce the following surface characteristics: high polarity (PEG methacrylate); electro-activity (vinyl ferrocene); fluorescence (eosin acrylate); thiol groups (pentaerythritol tetrakis(3-mercaptopropionate)). The method has broad applicability and can be used to produce multifunctional imprinted nanoparticles with potential for further application in the biosensors, diagnostics and biomedical fields and as an alternative to natural receptors.